| Description |
The Human Osteopontin (OPN) ELISA Kit quantities OPN levels in human serum, plasma, urine, or tissue homogenates. OPN is a secreted matricellular cytokine synthesized by cells involved in bone morphogenesis. It is highly up-regulated in an acute and chronic inflammatory environment. And it is implicated in physiological and pathophysiologic processes, including biomineralization and bone remodeling, cellular immunity and wound healing, and tumorigenesis. OPN has been demonstrated to promote proliferation, angiogenesis, and metastasis of tumor cells. It also signals through integrin and CD44 receptors. OPN interacts with CD44 via an RGD-independent manner to express different functional activities in chemotaxis of macrophages, cell adhesion of fibroblasts, and cytoprotection and regulation of T helper type 1 cells and bone marrow cells.
This kit employs the sandwich-ELISA mechanism in conjugation with OPN antibody-OPN antigen-specific binding as well as HRP-TMB chromogenic reaction to measure the concentrations of OPN in the samples. The kit is characterized by high sensitivity, strong specificity, good linearity, recovery with 81%-106%, precision less than 10%. Refer to the product instructions for more validation information.
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| Target Name |
secreted phosphoprotein 1 |
| Alternative Names |
BNSP ELISA Kit; Bone sialoprotein 1 ELISA Kit; Bone sialoprotein I ELISA Kit; BSP I ELISA Kit; BSPI ELISA Kit; Early T lymphocyte activation 1 ELISA Kit; ETA 1 ELISA Kit; ETA1 ELISA Kit; MGC110940 ELISA Kit; Nephropontin ELISA Kit; OPN ELISA Kit; Osteopontin ELISA Kit; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein ELISA Kit; OSTP_HUMAN ELISA Kit; PSEC0156 ELISA Kit; secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1) ELISA Kit; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) ELISA Kit; Secreted phosphoprotein 1 ELISA Kit; SPP 1 ELISA Kit; SPP-1 ELISA Kit; SPP1 ELISA Kit; SPP1/CALPHA1 fusion ELISA Kit; Urinary stone protein ELISA Kit; Uropontin ELISA Kit |
| Abbreviation |
OPN |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, urine, tissue homogenates |
| Detection Range |
0.78 ng/mL-50 ng/mL |
| Sensitivity |
0.195 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human OPN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
94 |
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| Range % |
88-98 |
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| 1:10 |
Average % |
100 |
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| Range % |
94-104 |
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| 1:20 |
Average % |
93 |
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| Range % |
88-97 |
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| 1:40 |
Average % |
99 |
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| Range % |
93-104 |
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| Recovery |
| The recovery of human OPN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
81-97 |
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| EDTA plasma (n=4) |
102 |
97-106 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 50 |
2.418 |
2.333 |
2.376 |
2.271 |
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| 25 |
1.684 |
1.706 |
1.695 |
1.590 |
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| 12.5 |
1.175 |
1.152 |
1.164 |
1.059 |
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| 6.25 |
0.654 |
0.664 |
0.659 |
0.554 |
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| 3.12 |
0.383 |
0.361 |
0.372 |
0.267 |
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| 1.56 |
0.245 |
0.232 |
0.239 |
0.134 |
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| 0.78 |
0.176 |
0.185 |
0.181 |
0.076 |
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| 0 |
0.102 |
0.108 |
0.105 |
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| Materials provided |
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human OPN antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled OPN antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
