Human P-Selectin ELISA kit
Product Details
| Target Name |
P-Selectin |
| Alternative Names |
Antigen CD62 ELISA Kit; CD62 ELISA Kit; CD62 antigen-like family member P ELISA Kit; CD62P ELISA Kit; GMP 140 ELISA Kit; GMP-140 ELISA Kit; GMP140 ELISA Kit; GMRP ELISA Kit; Granule membrane protein 140 ELISA Kit; Granulocyte membrane protein ELISA Kit; GRMP ELISA Kit; LECAM 3 ELISA Kit; LECAM3 ELISA Kit; Leukocyte endothelial cell adhesion molecule 3 ELISA Kit; Leukocyte-endothelial cell adhesion molecule 3 ELISA Kit; LYAM3_HUMAN ELISA Kit; P Selectin ELISA Kit; P-selectin ELISA Kit; PADGEM ELISA Kit; Platelet activation dependent granule-external membrane protein ELISA Kit; Platelet alpha granule membrane protein ELISA Kit; PSEL ELISA Kit; Selectin P (granule membrane protein 140kDa, antigen CD62) ELISA Kit; SELP ELISA Kit |
| Abbreviation |
SELP |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.9 ng/mL-60 ng/mL |
| Sensitivity |
0.225 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human P-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
86 |
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| Range % |
80-92 |
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| 1:10 |
Average % |
97 |
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| Range % |
91-105 |
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| 1:20 |
Average % |
98 |
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| Range % |
92-110 |
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| 1:40 |
Average % |
95 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of human P-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
97 |
90-104 |
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| EDTA plasma (n=4) |
96 |
87-102 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 60 |
2.305 |
2.368 |
2.337 |
2.269 |
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| 30 |
1.795 |
1.805 |
1.800 |
1.732 |
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| 15 |
1.146 |
1.187 |
1.167 |
1.099 |
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| 7.5 |
0.653 |
0.689 |
0.671 |
0.603 |
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| 3.75 |
0.344 |
0.358 |
0.351 |
0.283 |
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| 1.8 |
0.245 |
0.264 |
0.255 |
0.187 |
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| 0.9 |
0.110 |
0.116 |
0.113 |
0.045 |
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| 0 |
0.066 |
0.069 |
0.068 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
