Human Parathyroid Hormone Related Protein,PTHrP ELISA Kit
Product Details
| Target Name |
parathyroid hormone-like hormone |
| Alternative Names |
HHM ELISA Kit; MGC14611 ELISA Kit; Osteostatin ELISA Kit; Parathyroid hormon like hormone isoform 2 preproprotein ELISA Kit; Parathyroid hormone like hormone ELISA Kit; Parathyroid hormone like hormone isoform 1 preproprotein ELISA Kit; Parathyroid hormone like protein ELISA Kit; Parathyroid hormone like related protein ELISA Kit; Parathyroid hormone related protein ELISA Kit; Parathyroid hormone-like protein ELISA Kit; Parathyroid like protein ELISA Kit; PLP ELISA Kit; PTH related protein ELISA Kit; PTH-rP ELISA Kit; PTHLH ELISA Kit; PTHR ELISA Kit; PTHR_HUMAN ELISA Kit; PTHrP ELISA Kit; PTHrP[107-139] ELISA Kit |
| Abbreviation |
PTHLH |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.81 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human PTHrP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
94 |
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| Range % |
90-99 |
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| 1:2 |
Average % |
87 |
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| Range % |
82-92 |
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| 1:4 |
Average % |
96 |
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| Range % |
90-102 |
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| 1:8 |
Average % |
90 |
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| Range % |
85-96 |
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| Recovery |
| The recovery of human PTHrP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
89 |
84-94 |
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| EDTA plasma (n=4) |
100 |
95-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.401 |
2.458 |
2.430 |
2.332 |
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| 1000 |
1.763 |
1.788 |
1.776 |
1.678 |
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| 500 |
1.184 |
1.195 |
1.190 |
1.092 |
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| 250 |
0.821 |
0.885 |
0.853 |
0.755 |
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| 125 |
0.398 |
0.367 |
0.383 |
0.285 |
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| 62.5 |
0.228 |
0.221 |
0.225 |
0.127 |
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| 31.25 |
0.106 |
0.099 |
0.103 |
0.005 |
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| 0 |
0.095 |
0.101 |
0.098 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
