Human Pepsinogen I ELISA Kit
Product Details
| Target Name |
Pepsinogen I |
| Abbreviation |
PG I |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
6.25 ng/mL-400 ng/mL |
| Sensitivity |
1.56 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human pepsinogen I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
84 |
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| Range % |
80-92 |
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| 1:2 |
Average % |
106 |
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| Range % |
100-115 |
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| 1:4 |
Average % |
97 |
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| Range % |
94-112 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of human pepsinogen I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-99 |
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| EDTA plasma (n=4) |
97 |
90-102 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.396 |
2.252 |
2.324 |
2.207 |
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| 200 |
1.612 |
1.736 |
1.674 |
1.557 |
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| 100 |
1.104 |
1.054 |
1.079 |
0.962 |
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| 50 |
0.662 |
0.639 |
0.651 |
0.534 |
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| 25 |
0.399 |
0.424 |
0.412 |
0.295 |
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| 12.5 |
0.292 |
0.309 |
0.301 |
0.184 |
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| 6.25 |
0.205 |
0.199 |
0.202 |
0.085 |
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| 0 |
0.118 |
0.115 |
0.117 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
