Human peroxisome proliferative activated receptor gama coactivator 1 alpha,PPARGC1 ELISA Kit
Product Details
| Target Name |
peroxisome proliferator-activated receptor gamma, coactivator 1 alpha |
| Alternative Names |
L PGC 1alpha ELISA Kit; LEM6 ELISA Kit; Ligand effect modulator 6 ELISA Kit; Peroxisome proliferative activated receptor gamma coactivator 1 alpha ELISA Kit; Peroxisome proliferative activated receptor gamma coactivator 1 ELISA Kit; Peroxisome proliferator activated receptor gamma coactivator 1 alpha ELISA Kit; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 3ext ELISA Kit; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 8a ELISA Kit; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 ELISA Kit; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 ELISA Kit; Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 NT ELISA Kit; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha ELISA Kit; PGC 1 (alpha) ELISA Kit; PGC 1 alpha ELISA Kit; PGC 1v ELISA Kit; PGC-1-alpha ELISA Kit; PGC1 ELISA Kit; PGC1(alpha) ELISA Kit; PGC1A ELISA Kit; PGC1v ELISA Kit; PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6 ELISA Kit; PPAR gamma coactivator 1 alpha ELISA Kit; PPAR gamma coactivator 1 ELISA Kit; PPAR gamma coactivator variant form ELISA Kit; PPAR-gamma coactivator 1-alpha ELISA Kit; PPARGC 1 alpha ELISA Kit; PPARGC-1-alpha ELISA Kit; PPARGC1 ELISA Kit; PPARGC1A ELISA Kit; PRGC1_HUMAN ELISA Kit |
| Abbreviation |
PPARGC1A |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates, cell lysates |
| Detection Range |
125 pg/mL-8000 pg/mL |
| Sensitivity |
31.25 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human PPARGC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
84 |
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| Range % |
80-92 |
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| 1:2 |
Average % |
97 |
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| Range % |
90-105 |
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| 1:4 |
Average % |
99 |
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| Range % |
92-110 |
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| 1:8 |
Average % |
95 |
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| Range % |
86-99 |
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| Recovery |
| The recovery of human PPARGC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-100 |
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| EDTA plasma (n=4) |
96 |
90-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 8000 |
2.054 |
2.052 |
2.053 |
1.954 |
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| 4000 |
1.315 |
1.325 |
1.320 |
1.221 |
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| 2000 |
0.762 |
0.777 |
0.770 |
0.671 |
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| 1000 |
0.470 |
0.477 |
0.474 |
0.375 |
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| 500 |
0.284 |
0.295 |
0.290 |
0.191 |
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| 250 |
0.216 |
0.224 |
0.220 |
0.121 |
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| 125 |
0.157 |
0.165 |
0.161 |
0.062 |
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| 0 |
0.096 |
0.102 |
0.099 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
