Human Phosphatidylinositol-glycan-specific phospholipase D(GPLD1) ELISA kit
Product Details
| Target Name |
glycosylphosphatidylinositol specific phospholipase D1 |
| Alternative Names |
Glycoprotein phospholipase D ELISA Kit; Glycosyl-phosphatidylinositol-specific phospholipase D ELISA Kit; GPI-PLD ELISA Kit; GPI-specific phospholipase D ELISA Kit; GPIPLD ELISA Kit; GPIPLDM ELISA Kit; GPLD1 ELISA Kit; MGC22590 ELISA Kit; PHLD_HUMAN ELISA Kit; Phosphatidylinositol glycan specific phospholipase D ELISA Kit; Phosphatidylinositol-glycan-specific phospholipase D ELISA Kit; PI-G PLD ELISA Kit; PIGPLD ELISA Kit; PIGPLD1 ELISA Kit |
| Abbreviation |
GPLD1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
3.12 ng/mL-200 ng/mL |
| Sensitivity |
0.78 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human GPLD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:200 |
Average % |
90 |
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| Range % |
84-96 |
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| 1:400 |
Average % |
97 |
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| Range % |
91-103 |
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| 1:800 |
Average % |
103 |
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| Range % |
97-109 |
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| 1:1600 |
Average % |
89 |
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| Range % |
83-95 |
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| Recovery |
| The recovery of human GPLD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
100 |
94-106 |
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| EDTA plasma (n=4) |
95 |
91-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.841 |
2.752 |
2.797 |
2.673 |
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| 100 |
2.564 |
2.402 |
2.483 |
2.359 |
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| 50 |
2.135 |
1.965 |
2.050 |
1.926 |
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| 25 |
1.358 |
1.355 |
1.357 |
1.233 |
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| 12.5 |
0.691 |
0.744 |
0.718 |
0.594 |
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| 6.25 |
0.371 |
0.395 |
0.383 |
0.259 |
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| 3.12 |
0.225 |
0.226 |
0.226 |
0.102 |
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| 0 |
0.125 |
0.123 |
0.124 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
