Human phosphoglycerate kinase 1 (PGK1) ELISA kit
Product Details
| Target Name |
phosphoglycerate kinase 1 |
| Alternative Names |
Cell migration-inducing gene 10 protein ELISA Kit; Epididymis secretory sperm binding protein Li 68p ELISA Kit; HEL S 68p ELISA Kit; MGC117307 ELISA Kit; MGC8947 ELISA Kit; MIG10 ELISA Kit; pgk1 ELISA Kit; PGK1_HUMAN ELISA Kit; PGKA ELISA Kit; Phosphoglycerate kinase 1 ELISA Kit; Primer recognition protein 2 ELISA Kit; PRP 2 ELISA Kit |
| Abbreviation |
PGK1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
28 pg/mL-1800 pg/mL |
| Sensitivity |
7 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human PGK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
87-97 |
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| 1:2 |
Average % |
99 |
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| Range % |
95-104 |
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| 1:4 |
Average % |
101 |
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| Range % |
94-105 |
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| 1:8 |
Average % |
95 |
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| Range % |
89-99 |
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| Recovery |
| The recovery of human PGK1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
91 |
86-97 |
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| EDTA plasma (n=4) |
99 |
95-107 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 1800 |
2.894 |
3.011 |
2.953 |
2.762 |
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| 900 |
2.266 |
2.244 |
2.255 |
2.064 |
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| 450 |
1.393 |
1.474 |
1.434 |
1.243 |
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| 225 |
0.734 |
0.763 |
0.749 |
0.558 |
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| 112.5 |
0.497 |
0.480 |
0.489 |
0.298 |
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| 56 |
0.354 |
0.338 |
0.346 |
0.155 |
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| 28 |
0.267 |
0.277 |
0.272 |
0.081 |
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| 0 |
0.186 |
0.195 |
0.191 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
