Human Phospholipidase A2,PLA2 ELISA Kit
Product Details
| Target Name |
phospholipase A2, group IB (pancreas) |
| Alternative Names |
Group IB phospholipase A2 ELISA Kit; Group IB secretory phospholipase A2 ELISA Kit; MGC119834 ELISA Kit; MGC119835 ELISA Kit; PA21B_HUMAN ELISA Kit; Phosphatidylcholine 2 acylhydrolase ELISA Kit; Phosphatidylcholine 2-acylhydrolase 1B ELISA Kit; Phospholipase A2 ELISA Kit; Phospholipase A2 group IB (pancreas) ELISA Kit; Phospholipase A2 group IB ELISA Kit; PLA 2 ELISA Kit; PLA2 ELISA Kit; PLA2A ELISA Kit; PLA2G1B ELISA Kit; PPLA 2 ELISA Kit; PPLA2 ELISA Kit; sPLA2IB ELISA Kit |
| Abbreviation |
PLA2G1B |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.45 ng/mL-30 ng/mL |
| Sensitivity |
0.11 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human PLA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
93 |
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| Range % |
88-97 |
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| 1:10 |
Average % |
104 |
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| Range % |
100-109 |
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| 1:20 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:40 |
Average % |
85 |
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| Range % |
81-92 |
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| Recovery |
| The recovery of human PLA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
88-96 |
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| EDTA plasma (n=4) |
97 |
93-101 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 30 |
2.534 |
2.452 |
2.493 |
2.384 |
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| 15 |
1.652 |
1.576 |
1.614 |
1.505 |
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| 7.5 |
1.066 |
1.004 |
1.035 |
0.926 |
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| 3.75 |
0.578 |
0.561 |
0.570 |
0.461 |
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| 1.8 |
0.387 |
0.401 |
0.394 |
0.285 |
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| 0.9 |
0.238 |
0.245 |
0.242 |
0.133 |
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| 0.45 |
0.179 |
0.187 |
0.183 |
0.074 |
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| 0 |
0.105 |
0.112 |
0.109 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
