Principle of the Assay
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti PIEZO1 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti PIEZO1 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with PIEZO1 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of PIEZO1 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
