Human pigment epithelium-derived factor,PEDF ELISA Kit
Product Details
| Target Name |
pigment epithelium-derived factor,PEDF |
| Alternative Names |
Cell proliferation-inducing gene 35 protein ELISA Kit; EPC 1 ELISA Kit; EPC-1 ELISA Kit; EPC1 ELISA Kit; OI12 ELISA Kit; OI6 ELISA Kit; PEDF ELISA Kit; PEDF_HUMAN ELISA Kit; PIG 35 ELISA Kit; PIG35 ELISA Kit; Pigment epithelium derived factor ELISA Kit; Pigment epithelium-derived factor ELISA Kit; Proliferation inducing protein 35 ELISA Kit; Serine (or cysteine) proteinase inhibitor ELISA Kit; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 ELISA Kit; Serpin F1 ELISA Kit; Serpin family F member 1 ELISA Kit; Serpin peptidase inhibitor ELISA Kit; Serpin peptidase inhibitor clade F member 1 ELISA Kit; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 ELISA Kit; SERPINF 1 ELISA Kit; Serpinf1 ELISA Kit |
| Abbreviation |
PEDF |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, cell culture supernates, urine, tissue homogenates |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8>
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10>
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human PEDF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
100 |
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| Range % |
96-104 |
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| 1:2 |
Average % |
95 |
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| Range % |
89-104 |
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| 1:4 |
Average % |
96 |
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| Range % |
91-103 |
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| 1:8 |
Average % |
92 |
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| Range % |
88-97 |
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| Recovery |
| The recovery of human PEDF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
91 |
85-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.535 |
2.575 |
2.555 |
2.441 |
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| 5 |
2.012 |
2.032 |
2.022 |
1.908 |
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| 2.5 |
1.398 |
1.428 |
1.413 |
1.299 |
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| 1.25 |
0.847 |
0.867 |
0.857 |
0.743 |
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| 0.625 |
0.478 |
0.498 |
0.488 |
0.374 |
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| 0.312 |
0.336 |
0.346 |
0.341 |
0.227 |
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| 0.156 |
0.237 |
0.250 |
0.244 |
0.130 |
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| 0 |
0.109 |
0.118 |
0.114 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
