Human Plasma protease C1 inhibitor(SERPING1) ELISA kit
Product Details
| Target Name |
serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 |
| Alternative Names |
C1 esterase inhibitor ELISA Kit; C1 Inh ELISA Kit; C1 inhibiting factor ELISA Kit; C1 inhibitor ELISA Kit; C1-inhibiting factor ELISA Kit; C1IN ELISA Kit; C1Inh ELISA Kit; C1NH ELISA Kit; complement component 1 inhibitor ELISA Kit; esterase inhibitor ELISA Kit; HAE1 ELISA Kit; HAE2 ELISA Kit; IC1_HUMAN ELISA Kit; Plasma protease C1 inhibitor ELISA Kit; Serine (or cysteine) proteinase inhibitor clade G member 1 ELISA Kit; serine/cysteine proteinase inhibitor clade G member 1 ELISA Kit; Serpin G1 ELISA Kit; serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 ELISA Kit; SERPING1 ELISA Kit |
| Abbreviation |
SERPING1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
18.75 ng/mL-1200 ng/mL |
| Sensitivity |
4.7 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Blood Coagulation |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human SERPING1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:200 |
Average % |
99 |
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| Range % |
95-103 |
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| 1:400 |
Average % |
94 |
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| Range % |
91-98 |
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| 1:800 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:1600 |
Average % |
92 |
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| Range % |
85-96 |
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| Recovery |
| The recovery of human SERPING1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
88-99 |
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| EDTA plasma (n=4) |
89 |
82-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 1200 |
0.090 |
0.091 |
0.091 |
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| 600 |
0.115 |
0.117 |
0.116 |
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| 300 |
0.166 |
0.175 |
0.171 |
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| 150 |
0.272 |
0.261 |
0.267 |
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| 75 |
0.466 |
0.455 |
0.461 |
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| 37.5 |
0.784 |
0.773 |
0.779 |
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| 18.75 |
1.020 |
1.110 |
1.065 |
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| 0 |
2.103 |
2.205 |
2.154 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
