Human Plasminogen activator inhibitor 2(SERPINB2) ELISA kit
Product Details
| Target Name |
serpin peptidase inhibitor, clade B (ovalbumin), member 2 |
| Alternative Names |
HsT1201 ELISA Kit; Monocyte Arg serpin ELISA Kit; Monocyte Arg-serpin ELISA Kit; Monocyte Arginine-serpin ELISA Kit; Monocyte-derived plasminogen activator inhibitor ELISA Kit; PAI ELISA Kit; PAI-2 ELISA Kit; PAI2 ELISA Kit; PAI2_HUMAN ELISA Kit; Placental plasminogen activator inhibitor ELISA Kit; PLANH2 ELISA Kit; Plasminogen activator inhibitor 2 ELISA Kit; plasminogen activator inhibitor; type 2 ELISA Kit; plasminogen activator inhibitor; type II (arginine-serpin) ELISA Kit; serine (or cysteine) proteinase inhibitor; clade B (ovalbumin); member 2 ELISA Kit; Serpin B2 ELISA Kit; serpin peptidase inhibitor; clade B (ovalbumin); member 2 ELISA Kit; Serpinb2 ELISA Kit; Urokinase inhibitor ELISA Kit |
| Abbreviation |
SERPINB2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates, cell lysates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cell Biology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human SERPINB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
|
| 1:200 |
Average % |
96 |
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| Range % |
90-102 |
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| 1:400 |
Average % |
98 |
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| Range % |
93-104 |
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| 1:800 |
Average % |
95 |
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| Range % |
89-99 |
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| 1:1600 |
Average % |
88 |
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| Range % |
84-92 |
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| Recovery |
| The recovery of human SERPINB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
99 |
95-103 |
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| EDTA plasma (n=4) |
86 |
82-92 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 1000 |
2.687 |
2.521 |
2.604 |
2.455 |
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| 500 |
2.126 |
2.235 |
2.181 |
2.032 |
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| 250 |
1.586 |
1.573 |
1.580 |
1.431 |
|
| 125 |
0.972 |
0.995 |
0.984 |
0.835 |
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| 62.5 |
0.617 |
0.606 |
0.612 |
0.463 |
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| 31.25 |
0.378 |
0.365 |
0.372 |
0.223 |
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| 15.6 |
0.232 |
0.237 |
0.235 |
0.086 |
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| 0 |
0.149 |
0.148 |
0.149 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
