Human Platelet Factor 4,PF-4 ELISA Kit
Product Details
| Target Name |
platelet factor 4 |
| Alternative Names |
C-X-C motif chemokine 4 ELISA Kit; Chemokine (C X C motif) ligand 4 ELISA Kit; Chemokine (CXC motif) ligand 4 ELISA Kit; chemokine ligand 4 ELISA Kit; CXCL 4 ELISA Kit; CXCL4 ELISA Kit; Iroplact ELISA Kit; MGC138298 ELISA Kit; Oncostatin A ELISA Kit; Oncostatin-A ELISA Kit; OncostatinA ELISA Kit; PF 4 ELISA Kit; PF-4 ELISA Kit; PF4 ELISA Kit; Pf4a ELISA Kit; Platelet factor 4 ELISA Kit; PLF4_HUMAN ELISA Kit; SCYB 4 ELISA Kit; SCYB4 ELISA Kit; short form ELISA Kit; Small inducible cytokine subfamily B ELISA Kit; Small inducible cytokine subfamily member 4 ELISA Kit |
| Abbreviation |
PF4 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
6.25 ng/mL-400 ng/mL |
| Sensitivity |
1.56 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human PF-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:20 |
Average % |
95 |
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| Range % |
92-101 |
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| 1:40 |
Average % |
93 |
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| Range % |
83-99 |
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| 1:80 |
Average % |
95 |
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| Range % |
90-100 |
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| Recovery |
| The recovery of human PF-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
86-97 |
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| EDTA plasma (n=4) |
96 |
90-101 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.548 |
2.452 |
2.500 |
2.294 |
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| 200 |
2.196 |
2.076 |
2.136 |
1.930 |
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| 100 |
1.632 |
1.593 |
1.613 |
1.407 |
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| 50 |
1.082 |
1.122 |
1.102 |
0.896 |
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| 25 |
0.675 |
0.645 |
0.660 |
0.454 |
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| 12.5 |
0.441 |
0.436 |
0.439 |
0.233 |
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| 6.25 |
0.320 |
0.315 |
0.318 |
0.112 |
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| 0 |
0.205 |
0.207 |
0.206 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
