Human Pro Atrial Natriuretic Peptide,Pro-ANP ELISA Kit
Product Details
| Target Name |
Pro Atrial Natriuretic Peptide,Pro-ANP |
| Abbreviation |
Pro-ANP |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
62.5 pg/mL-4000 pg/mL |
| Sensitivity |
15.6 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human Pro-ANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
86-97 |
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| 1:2 |
Average % |
85 |
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| Range % |
80-89 |
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| 1:4 |
Average % |
92 |
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| Range % |
87-98 |
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| 1:8 |
Average % |
104 |
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| Range % |
96-108 |
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| Recovery |
| The recovery of human Pro-ANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
84-95 |
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| EDTA plasma (n=4) |
87 |
82-93 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 4000 |
1.943 |
1.896 |
1.920 |
1.766 |
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| 2000 |
1.452 |
1.366 |
1.409 |
1.255 |
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| 1000 |
0.998 |
0.968 |
0.983 |
0.829 |
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| 500 |
0.646 |
0.657 |
0.652 |
0.498 |
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| 250 |
0.467 |
0.454 |
0.461 |
0.307 |
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| 125 |
0.322 |
0.306 |
0.314 |
0.160 |
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| 62.5 |
0.209 |
0.203 |
0.206 |
0.052 |
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| 0 |
0.155 |
0.153 |
0.154 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
