Human Profilin 1 ELISA Kit
Product Details
| Target Name |
profilin 1 |
| Alternative Names |
Actin binding protein ELISA Kit; ALS18 ELISA Kit; Epididymis tissue protein Li 184a ELISA Kit; OTTHUMP00000125244 ELISA Kit; PFN 1 ELISA Kit; Pfn ELISA Kit; PFN1 ELISA Kit; PROF1_HUMAN ELISA Kit; Profilin I ELISA Kit; Profilin-1 ELISA Kit; Profilin1 ELISA Kit; ProfilinI ELISA Kit |
| Abbreviation |
PFN1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, urine |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human profilin 1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
86 |
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| Range % |
80-92 |
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| 1:2 |
Average % |
93 |
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| Range % |
90-100 |
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| 1:4 |
Average % |
97 |
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| Range % |
92-105 |
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| 1:8 |
Average % |
93 |
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| Range % |
85-98 |
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| Recovery |
| The recovery of human profilin 1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
98 |
92-106 |
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| EDTA plasma (n=4) |
96 |
92-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.139 |
2.002 |
2.071 |
1.900 |
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| 1000 |
1.925 |
1.776 |
1.851 |
1.680 |
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| 500 |
1.613 |
1.604 |
1.609 |
1.438 |
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| 250 |
1.254 |
1.189 |
1.222 |
1.051 |
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| 125 |
0.848 |
0.783 |
0.816 |
0.645 |
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| 62.5 |
0.529 |
0.509 |
0.519 |
0.348 |
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| 31.25 |
0.331 |
0.311 |
0.321 |
0.150 |
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| 0 |
0.172 |
0.169 |
0.171 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
