Human Programmed cell death 1 ligand 2(PDCD1LG2) ELISA kit
Product Details
| Target Name |
programmed cell death 1 ligand 2 |
| Alternative Names |
B7 dendritic cell molecule ELISA Kit; B7-DC ELISA Kit; B7DC ELISA Kit; bA574F11.2 ELISA Kit; Btdc ELISA Kit; Butyrophilin B7 DC ELISA Kit; Butyrophilin B7-DC ELISA Kit; Butyrophilin B7DC ELISA Kit; CD 273 ELISA Kit; CD273 ELISA Kit; CD273 antigen ELISA Kit; MGC142238 ELISA Kit; MGC142240 ELISA Kit; PD 1 ligand 2 ELISA Kit; PD L2 ELISA Kit; PD-1 ligand 2 ELISA Kit; PD-L2 ELISA Kit; PD1 ligand 2 ELISA Kit; PD1L2_HUMAN ELISA Kit; PDCD 1 ligand 2 ELISA Kit; PDCD1 ligand 2 ELISA Kit; PDCD1L2 ELISA Kit; Pdcd1lg2 ELISA Kit; PDL 2 ELISA Kit; PDL2 ELISA Kit; Programmed cell death 1 ligand 2 ELISA Kit; Programmed death ligand 2 ELISA Kit |
| Abbreviation |
PDCD1LG2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
46.88 pg/mL-3000 pg/mL |
| Sensitivity |
11.72 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human PDCD1LG2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
86-97 |
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| 1:2 |
Average % |
97 |
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| Range % |
92-103 |
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| 1:4 |
Average % |
101 |
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| Range % |
97-105 |
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| 1:8 |
Average % |
89 |
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| Range % |
83-95 |
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| Recovery |
| The recovery of human PDCD1LG2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
90 |
85-93 |
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| EDTA plasma (n=4) |
101 |
96-104 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 3000 |
2.788 |
2.899 |
2.844 |
2.745 |
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| 1500 |
2.365 |
2.456 |
2.411 |
2.312 |
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| 750 |
1.545 |
1.636 |
1.591 |
1.492 |
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| 375 |
0.778 |
0.745 |
0.762 |
0.663 |
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| 187.5 |
0.423 |
0.411 |
0.417 |
0.318 |
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| 93.75 |
0.256 |
0.265 |
0.261 |
0.162 |
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| 46.88 |
0.155 |
0.164 |
0.160 |
0.061 |
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| 0 |
0.101 |
0.097 |
0.099 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
