Human Programmed Death 1(PD-1)ELISA KIT
Product Details
| Target Name |
programmed cell death 1 |
| Alternative Names |
CD279 ELISA Kit; CD279 antigen ELISA Kit; hPD 1 ELISA Kit; hPD l ELISA Kit; hPD-1 ELISA Kit; hSLE1 ELISA Kit; PD 1 ELISA Kit; PD-1 ELISA Kit; PD1 ELISA Kit; PDCD 1 ELISA Kit; PDCD1 ELISA Kit; PDCD1_HUMAN ELISA Kit; Programmed cell death 1 ELISA Kit; Programmed cell death 1 protein ELISA Kit; Programmed cell death protein 1 ELISA Kit; Protein PD 1 ELISA Kit; Protein PD-1 ELISA Kit; SLEB2 ELISA Kit; Systemic lupus erythematosus susceptibility 2 ELISA Kit |
| Abbreviation |
PDCD1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cell Biology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human PD-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
88-98 |
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| 1:2 |
Average % |
99 |
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| Range % |
95-104 |
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| 1:4 |
Average % |
95 |
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| Range % |
87-99 |
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| 1:8 |
Average % |
90 |
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| Range % |
86-94 |
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| Recovery |
| The recovery of human PD-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
100 |
96-104 |
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| EDTA plasma (n=4) |
91 |
87-95 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 1000 |
2.673 |
2.617 |
2.645 |
2.437 |
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| 500 |
2.302 |
2.239 |
2.271 |
2.063 |
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| 250 |
1.853 |
1.714 |
1.784 |
1.576 |
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| 125 |
1.524 |
1.429 |
1.477 |
1.269 |
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| 62.5 |
1.057 |
0.996 |
1.027 |
0.819 |
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| 31.2 |
0.639 |
0.682 |
0.661 |
0.453 |
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| 15.6 |
0.307 |
0.329 |
0.318 |
0.110 |
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| 0 |
0.205 |
0.210 |
0.208 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
