human prosaposin(PSAP)Elisa kit
Product Details
| Target Name |
prosaposin |
| Alternative Names |
A1 activator ELISA Kit; Cerebroside sulfate activator ELISA Kit; Co-beta-glucosidase ELISA Kit; Component C ELISA Kit; CSAct ELISA Kit; Dispersin ELISA Kit; GLBA ELISA Kit; Glucosylceramidase activator ELISA Kit; Proactivator polypeptide ELISA Kit; Proactivator polypeptide precursor ELISA Kit; Prosaposin (sphingolipid activator protein 1) ELISA Kit; prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy) ELISA Kit; Prosaposin ELISA Kit; Protein A ELISA Kit; Protein C ELISA Kit; PSAP ELISA Kit; SAP 1 ELISA Kit; SAP 2 ELISA Kit; SAP-1 ELISA Kit; SAP-2 ELISA Kit; SAP_HUMAN ELISA Kit; SAP1 ELISA Kit; Saposin A ELISA Kit; Saposin B ELISA Kit; Saposin B Val ELISA Kit; Saposin C ELISA Kit; Saposin D ELISA Kit; Saposin-D ELISA Kit; Saposins ELISA Kit; Sgp1 ELISA Kit; Sphingolipid activator protein 1 ELISA Kit; Sphingolipid activator protein 2 ELISA Kit; Sulfated glycoprotein 1 ELISA Kit; Sulfatide/GM1 activator ELISA Kit |
| Abbreviation |
PSAP |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.625 ng/mL-20 ng/mL |
| Sensitivity |
0.312 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human PSAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
101 |
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| Range % |
92-105 |
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| 1:2 |
Average % |
88 |
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| Range % |
82-91 |
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| 1:4 |
Average % |
103 |
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| Range % |
98-107 |
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| 1:8 |
Average % |
96 |
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| Range % |
90-100 |
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| Recovery |
| The recovery of human PSAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
87-97 |
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| EDTA plasma (n=4) |
87 |
82-93 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 20 |
2.226 |
2.105 |
2.166 |
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| 10 |
1.347 |
1.323 |
1.335 |
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| 5 |
0.659 |
0.666 |
0.663 |
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| 1.25 |
0.193 |
0.201 |
0.197 |
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| 0.625 |
0.100 |
0.105 |
0.103 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
