Human Prostaglandin E2,PG-E2 ELISA Kit
Product Details
| Target Name |
Prostaglandin E2,PG-E2 |
| Abbreviation |
PG-E2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
1.5 pg/mL-300 pg/mL |
| Sensitivity |
0.75 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<15>
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15>
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:2 |
Average % |
92 |
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| Range % |
86-96 |
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| 1:4 |
Average % |
97 |
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| Range % |
92-103 |
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| 1:8 |
Average % |
84 |
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| Range % |
80-88 |
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| Recovery |
| The recovery of human PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
89-98 |
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| EDTA plasma (n=4) |
90 |
83-94 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 300 |
0.193 |
0.201 |
0.197 |
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| 75 |
0.349 |
0.327 |
0.338 |
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| 24 |
0.523 |
0.531 |
0.527 |
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| 6 |
0.903 |
0.962 |
0.933 |
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| 1.5 |
1.316 |
1.393 |
1.355 |
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| 0 |
2.271 |
2.212 |
2.242 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
