Human Prostasin(PRSS8) ELISA kit
Product Details
| Target Name |
protease, serine, 8 |
| Alternative Names |
2410039E18Rik ELISA Kit; AI313909 ELISA Kit; C79772 ELISA Kit; CAP1 ELISA Kit; Channel Activating Protease 1 ELISA Kit; mCAP1 ELISA Kit; Prostasin ELISA Kit; Prostasin heavy chain ELISA Kit; protease, serine, 8 ELISA Kit; Prss8 ELISA Kit; PRSS8_HUMAN ELISA Kit; Serine protease 8 ELISA Kit |
| Abbreviation |
PRSS8 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, urine, saliva, tissue homogenates |
| Detection Range |
2.35 ng/mL-150 ng/mL |
| Sensitivity |
0.58 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cell Biology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8>
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10>
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human PRSS8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:200 |
Average % |
84 |
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| Range % |
80-92 |
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| 1:400 |
Average % |
95 |
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| Range % |
91-105 |
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| 1:800 |
Average % |
97 |
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| Range % |
92-108 |
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| 1:1600 |
Average % |
93 |
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| Range % |
86-100 |
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| Recovery |
| The recovery of human PRSS8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
89-98 |
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| EDTA plasma (n=4) |
97 |
85-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 150 |
2.800 |
2.790 |
2.795 |
2.650 |
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| 75 |
2.309 |
2.298 |
2.304 |
2.159 |
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| 37.5 |
1.281 |
1.300 |
1.291 |
1.146 |
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| 18.75 |
0.650 |
0.698 |
0.674 |
0.529 |
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| 9.4 |
0.454 |
0.493 |
0.474 |
0.329 |
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| 4.7 |
0.251 |
0.270 |
0.261 |
0.116 |
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| 2.35 |
0.174 |
0.168 |
0.171 |
0.026 |
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| 0 |
0.144 |
0.146 |
0.145 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
