Human Protein Induced Vitamin K Absence or Antagonist-II(PIVKA-II) ELISA Kit
Product Details
| Target Name |
Protein Induced Vitamin K Absence or Antagonist-II(PIVKA-II) |
| Abbreviation |
PIVKA-II |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, ascitic fluid, tissue homogenates |
| Detection Range |
0.312 ng/mL-20 ng/mL |
| Sensitivity |
0.078 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human PIVKA-II in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
94 |
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| Range % |
90-101 |
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| 1:2 |
Average % |
97 |
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| Range % |
86-101 |
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| 1:4 |
Average % |
96 |
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| Range % |
90-108 |
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| 1:8 |
Average % |
94 |
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| Range % |
82-103 |
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| Recovery |
| The recovery of human PIVKA-II spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
90-104 |
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| EDTA plasma (n=4) |
97 |
91-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.467 |
2.418 |
2.443 |
2.327 |
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| 10 |
1.962 |
1.945 |
1.954 |
1.838 |
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| 5 |
1.342 |
1.311 |
1.327 |
1.211 |
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| 2.5 |
0.823 |
0.772 |
0.798 |
0.682 |
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| 1.25 |
0.504 |
0.496 |
0.500 |
0.384 |
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| 0.625 |
0.331 |
0.321 |
0.326 |
0.210 |
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| 0.312 |
0.201 |
0.189 |
0.195 |
0.079 |
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| 0 |
0.117 |
0.114 |
0.116 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
