Human Protein NOV homolog(NOV) ELISA kit
Product Details
| Target Name |
nephroblastoma overexpressed gene |
| Alternative Names |
CCN family member 3 ELISA Kit; CCN3 ELISA Kit; IBP-9 ELISA Kit; IGF-binding protein 9 ELISA Kit; IGFBP-9 ELISA Kit; IGFBP9 ELISA Kit; Insulin-like growth factor-binding protein 9 ELISA Kit; Nephroblastoma overexpressed ELISA Kit; Nephroblastoma overexpressed gene protein homolog ELISA Kit; Nephroblastoma-overexpressed gene protein homolog ELISA Kit; NOV ELISA Kit; NOV_HUMAN ELISA Kit; NovH ELISA Kit; Protein NOV homolog ELISA Kit |
| Abbreviation |
NOV |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates_x000D_ |
| Detection Range |
62.5 pg/mL-4000 pg/mL |
| Sensitivity |
15.6 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human NOV in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
95-110 |
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| 1:2 |
Average % |
103 |
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| Range % |
99-112 |
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| 1:4 |
Average % |
92 |
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| Range % |
88-98 |
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| 1:8 |
Average % |
98 |
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| Range % |
93-104 |
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| Recovery |
| The recovery of human NOV spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
84-93 |
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| EDTA plasma (n=4) |
93 |
88-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 500 |
2.422 |
2.396 |
2.409 |
2.292 |
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| 250 |
1.692 |
1.642 |
1.667 |
1.550 |
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| 125 |
1.110 |
1.094 |
1.102 |
0.985 |
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| 62.5 |
0.713 |
0.689 |
0.701 |
0.584 |
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| 31.2 |
0.434 |
0.401 |
0.418 |
0.301 |
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| 15.6 |
0.347 |
0.353 |
0.350 |
0.233 |
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| 7.8 |
0.208 |
0.205 |
0.207 |
0.090 |
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| 0 |
0.120 |
0.114 |
0.117 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
