Human Protein Wnt-3a(WNT3A) ELISA kit
Product Details
| Description |
The product CSB-EL026136HU is a ready-to-use ELISA kit specifically designed and validated for the quantitative detection of human WNT3A in the serum and plasma. It is not intended for diagnostic use. This assay kit was designed and optimized for stem cell research use in humans. The kit has undergone rigorous quality control in multiple parameters, including sensitivity, specificity, precision, linearity, recovery, and inter-batch difference. Refer to the product instructions for more details. This assay employs the quantitative sandwich enzyme immunoassay technique, in which WNT3A in the samples or standards are sandwiched between pre-coated WNT3A antibody and Biotin-conjugated WNT3A antibody. HRP-avidin is then added to the wells. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of WNT3A bound in the initial step. The color development is stopped upon adding the stop solution, and the inte... Read more |
| Target Name |
wingless-type MMTV integration site family, member 3A |
| Alternative Names |
Protein Wnt 3a Precursor ELISA Kit; Protein Wnt-3a ELISA Kit; Wingless type MMTV integration site family member 3A ELISA Kit; Wnt 3a ELISA Kit; wnt3a ELISA Kit; WNT3A protein ELISA Kit; WNT3A_HUMAN ELISA Kit |
| Abbreviation |
WNT3A |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Stem Cells |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human WNT3A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
88 |
|
| Range % |
80-98 |
|
| 1:2 |
Average % |
95 |
|
| Range % |
90-100 |
|
| 1:4 |
Average % |
102 |
|
| Range % |
91-110 |
|
| 1:8 |
Average % |
92 |
|
| Range % |
86-98 |
|
|
| Recovery |
| The recovery of human WNT3A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
85-98 |
|
| EDTA plasma (n=4) |
96 |
90-103 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 10 |
2.030 |
1.952 |
1.991 |
1.823 |
|
| 5 |
1.549 |
1.476 |
1.513 |
1.345 |
|
| 2.5 |
1.092 |
1.014 |
1.053 |
0.885 |
|
| 1.25 |
0.712 |
0.709 |
0.711 |
0.543 |
|
| 0.625 |
0.487 |
0.449 |
0.468 |
0.300 |
|
| 0.312 |
0.372 |
0.359 |
0.366 |
0.198 |
|
| 0.156 |
0.273 |
0.254 |
0.264 |
0.096 |
|
| 0 |
0.170 |
0.166 |
0.168 |
|
|
|
|
| Materials provided |
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human WNT3A antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled WNT3A antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-conjugateDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
|
| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
