| Description |
This Human Protein Wnt-5a (WNT5A) ELISA kit is suitable for qualitatively determining WNT5A concentrations in multiple biological fluids, including in human serum, plasma, and tissue homogenates in vitro. WNT5A is a highly evolutionary conserved non-canonical WNT ligand that activates the non-canonical Wnt pathway. WNT5A signaling plays a critical role in the modulation of normal developmental processes, including proliferation, differentiation, migration, adhesion, and polarity. And it also participates in the regulation of innate immune response and promotes low-grade inflammation. In cancer, it acts as a tumor suppressor or promotes cancer cell invasion, migration, epithelial-mesenchymal transition (EMT), and metastasis. This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of human WNT5A in the sample. Standards and samples are respectively added to the microplate wells pre-coated with anti-human WNT5A antibody. Biotin-labeled WNT5A antibo... Read more |
| Target Name |
wingless-type MMTV integration site family, member 5A |
| Alternative Names |
hWNT 5A ELISA Kit; hWNT5A ELISA Kit; Protein Wnt 5a ELISA Kit; Protein Wnt-5a ELISA Kit; Protein Wnt5a ELISA Kit; Wingless type MMTV integration site family member 5A ELISA Kit; Wnt 5a ELISA Kit; WNT 5A protein ELISA Kit; WNT 5A protein precursor ELISA Kit; WNT5A ELISA Kit; WNT5A protein precursor ELISA Kit; WNT5A_HUMAN ELISA Kit |
| Abbreviation |
WNT5A |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Stem Cells |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human WNT5A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
90-95 |
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| 1:2 |
Average % |
95 |
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| Range % |
90-103 |
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| 1:4 |
Average % |
84 |
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| Range % |
80-92 |
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| 1:8 |
Average % |
100 |
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| Range % |
98-104 |
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| Recovery |
| The recovery of human WNT5A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
83-99 |
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| EDTA plasma (n=4) |
97 |
91-104 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
1.839 |
1.899 |
1.869 |
1.685 |
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| 5 |
1.571 |
1.583 |
1.577 |
1.393 |
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| 2.5 |
1.166 |
1.205 |
1.186 |
1.002 |
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| 1.25 |
0.732 |
0.781 |
0.757 |
0.573 |
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| 0.625 |
0.448 |
0.462 |
0.455 |
0.271 |
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| 0.312 |
0.325 |
0.341 |
0.333 |
0.149 |
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| 0.156 |
0.236 |
0.247 |
0.242 |
0.058 |
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| 0 |
0.183 |
0.185 |
0.184 |
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| Materials provided |
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human WNT5A antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled WNT5A antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
