Human proteinase 3(PR3) ELISA kit
Product Details
| Target Name |
proteinase 3 |
| Alternative Names |
ACPA ELISA Kit; AGP 7 ELISA Kit; AGP7 ELISA Kit; AGP7 serine proteinase ELISA Kit; Azurophil Granule Protein 7 ELISA Kit; C ANCA ELISA Kit; C ANCA antigen ELISA Kit; C-ANCA antigen ELISA Kit; CANCA ELISA Kit; EC 3.4.21.76 ELISA Kit; Leukocyte proteinase 3 ELISA Kit; MBN ELISA Kit; MBT ELISA Kit; MBT WEGENER AUTOANTIGEN ELISA Kit; Myeloblastin ELISA Kit; Neutrophil proteinase 4 ELISA Kit; NP 4 ELISA Kit; NP-4 ELISA Kit; NP4 ELISA Kit; P29 ELISA Kit; PR 3 ELISA Kit; PR-3 ELISA Kit; PR3 ELISA Kit; Proteinase 3 ELISA Kit; Proteinase3 ELISA Kit; PRTN 3 ELISA Kit; Prtn3 ELISA Kit; PRTN3_HUMAN ELISA Kit; Serine proteinase neutrophil Wegener granulomatosis autoantigen ELISA Kit; Serine proteinase; neutrophil ELISA Kit; Wegener autoantigen ELISA Kit; Wegener granulomatosis autoantigen ELISA Kit |
| Abbreviation |
PRTN3 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, tissue homogenates, plasma, urine |
| Detection Range |
0.78 ng/mL-50 ng/mL |
| Sensitivity |
0.195 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human PR3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
90 |
|
| Range % |
86-95 |
|
| 1:2 |
Average % |
88 |
|
| Range % |
81-93 |
|
| 1:4 |
Average % |
98 |
|
| Range % |
93-102 |
|
| 1:8 |
Average % |
102 |
|
| Range % |
98-106 |
|
|
| Recovery |
| The recovery of human PR3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
104 |
94-110 |
|
| EDTA plasma (n=4) |
92 |
88-97 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 50 |
2.002 |
2.050 |
2.026 |
1.934 |
|
| 25 |
1.503 |
1.584 |
1.544 |
1.452 |
|
| 12.5 |
1.014 |
1.101 |
1.058 |
0.966 |
|
| 6.25 |
0.598 |
0.606 |
0.602 |
0.510 |
|
| 3.12 |
0.384 |
0.387 |
0.386 |
0.294 |
|
| 1.56 |
0.295 |
0.301 |
0.298 |
0.206 |
|
| 0.78 |
0.197 |
0.189 |
0.193 |
0.101 |
|
| 0 |
0.095 |
0.089 |
0.092 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
