Human Pulmonary surfactant-associated protein C,SP-C ELISA Kit
Product Details
| Alternative Names |
BRICD6 ELISA Kit; BRICHOS domain containing 6 ELISA Kit; PSP C ELISA Kit; PSPC ELISA Kit; PSPC_HUMAN ELISA Kit; Pulmonary surfactant apoprotein 2 ELISA Kit; Pulmonary surfactant apoprotein PSP C ELISA Kit; pulmonary surfactant apoprotein-2 SP-C ELISA Kit; Pulmonary surfactant associated protein C ELISA Kit; Pulmonary surfactant associated proteolipid SPL pVal ELISA Kit; Pulmonary surfactant associated proteolipid SPL(Val) ELISA Kit; Pulmonary surfactant protein SP5 ELISA Kit; Pulmonary surfactant-associated protein C ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Val) ELISA Kit; SFTP 2 ELISA Kit; SFTP2 ELISA Kit; SFTPC ELISA Kit; SFTPC surfactant pulmonary associated protein C ELISA Kit; SMDP2 ELISA Kit; SP 5 ELISA Kit; SP C ELISA Kit; SP-C ELISA Kit; SP5 ELISA Kit; SPC ELISA Kit; Surfactant associated protein pulmonary 2 ELISA Kit; Surfactant protein c ELISA Kit; Surfactant proteolipid SPL-pVal ELISA Kit; Surfactant pulmonary associated protein C ELISA Kit |
| Abbreviation |
SFTPC |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates, urine |
| Detection Range |
9.38 pg/mL-600 pg/mL |
| Sensitivity |
2.3 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human SP-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:200 |
Average % |
92 |
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| Range % |
89-99 |
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| 1:400 |
Average % |
97 |
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| Range % |
91-101 |
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| 1:800 |
Average % |
98 |
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| Range % |
92-105 |
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| 1:1600 |
Average % |
96 |
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| Range % |
86-100 |
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| Recovery |
| The recovery of human SP-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
96 |
89-99 |
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| EDTA plasma (n=4) |
98 |
90-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 600 |
2.483 |
2.289 |
2.386 |
2.304 |
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| 300 |
1.876 |
1.772 |
1.824 |
1.742 |
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| 150 |
1.270 |
1.198 |
1.234 |
1.152 |
|
| 75 |
0.746 |
0.719 |
0.733 |
0.651 |
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| 37.5 |
0.512 |
0.501 |
0.507 |
0.425 |
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| 18.7 |
0.288 |
0.283 |
0.286 |
0.204 |
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| 9.37 |
0.211 |
0.209 |
0.210 |
0.128 |
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| 0 |
0.082 |
0.081 |
0.082 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
