Human Putative humanin peptide(MT-RNR2) ELISA kit
Product Details
| Target Name |
mitochondrially encoded 16S RNA |
| Alternative Names |
MT-RNR2 ELISA Kit; HN ELISA Kit; Humanin ELISA Kit; Humanin mitochondrial ELISA Kit; HNM ELISA Kit |
| Abbreviation |
MT-RNR2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
28 pg/mL-1800 pg/mL |
| Sensitivity |
7 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human MT-RNR2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
100 |
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| Range % |
92-105 |
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| 1:10 |
Average % |
97 |
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| Range % |
91-105 |
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| 1:20 |
Average % |
97 |
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| Range % |
92-106 |
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| 1:40 |
Average % |
93 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of human MT-RNR2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
85-99 |
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| EDTA plasma (n=4) |
95 |
90-101 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1800 |
2.120 |
2.163 |
2.142 |
2.007 |
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| 900 |
1.675 |
1.605 |
1.640 |
1.505 |
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| 450 |
1.158 |
1.168 |
1.163 |
1.028 |
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| 225 |
0.741 |
0.780 |
0.761 |
0.626 |
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| 112.5 |
0.352 |
0.383 |
0.368 |
0.233 |
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| 56.25 |
0.231 |
0.241 |
0.236 |
0.101 |
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| 28 |
0.178 |
0.186 |
0.182 |
0.047 |
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| 0 |
0.132 |
0.138 |
0.135 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
