Human RE1-silencing transcription factor(REST) ELISA kit
Product Details
| Target Name |
RE1-silencing transcription factor |
| Alternative Names |
Neural Restrictive Silencer Factor ELISA Kit; Neural-restrictive silencer factor ELISA Kit; Neuron Restrictive Silencer Factor ELISA Kit; NRSF ELISA Kit; RE1 silencing transcription factor ELISA Kit; RE1-silencing transcription factor ELISA Kit; Repressor binding to the X2 box ELISA Kit; rest ELISA Kit; REST protein ELISA Kit; REST_HUMAN ELISA Kit; X2 box repressor ELISA Kit; XBR ELISA Kit |
| Abbreviation |
REST |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.81 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Epigenetics and Nuclear Signaling |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human REST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
87 |
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| Range % |
80-92 |
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| 1:2 |
Average % |
96 |
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| Range % |
90-105 |
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| 1:4 |
Average % |
100 |
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| Range % |
91-110 |
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| 1:8 |
Average % |
92 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of human REST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
89-98 |
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| EDTA plasma (n=4) |
99 |
92-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
1.983 |
1.882 |
1.933 |
1.833 |
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| 1000 |
1.808 |
1.796 |
1.802 |
1.702 |
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| 500 |
1.636 |
1.604 |
1.620 |
1.520 |
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| 250 |
1.321 |
1.290 |
1.306 |
1.206 |
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| 125 |
0.918 |
0.909 |
0.914 |
0.814 |
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| 62.5 |
0.610 |
0.590 |
0.600 |
0.500 |
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| 31.25 |
0.323 |
0.318 |
0.321 |
0.221 |
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| 0 |
0.103 |
0.097 |
0.100 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
