Human receptor-binding cancer antigen expressed on SiSo cells，RCAS1 ELISA kit
Product Details
| Target Name |
estrogen receptor binding site associated, antigen, 9 |
| Alternative Names |
BAG9 ELISA Kit; Cancer associated surface antigen ELISA Kit; Cancer associated surface antigen RCAS1 ELISA Kit; Cancer-associated surface antigen RCAS1 ELISA Kit; EB9 ELISA Kit; EBAG 9 ELISA Kit; EBAG9 ELISA Kit; Estrogen receptor binding fragment associated gene 9 protein ELISA Kit; Estrogen receptor binding site associated antigen 9 ELISA Kit; estrogen receptor binding site associated; antigen; 9 ELISA Kit; Estrogen receptor-binding fragment-associated gene 9 protein ELISA Kit; PDAF ELISA Kit; RCAS 1 ELISA Kit; RCAS1 ELISA Kit; RCAS1_HUMAN ELISA Kit; Receptor binding cancer antigen expressed on SiSo cells ELISA Kit; Receptor-binding cancer antigen expressed on SiSo cells ELISA Kit |
| Abbreviation |
EBAG9 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, tissue homogenates |
| Detection Range |
0.78 U/mL-50 U/mL |
| Sensitivity |
0.195 U/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human RCAS1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
94 |
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| Range % |
90-98 |
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| 1:2 |
Average % |
96 |
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| Range % |
91-101 |
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| 1:4 |
Average % |
100 |
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| Range % |
95-106 |
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| 1:8 |
Average % |
105 |
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| Range % |
99-111 |
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| Recovery |
| The recovery of human RCAS1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
96 |
92-102 |
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| EDTA plasma (n=4) |
100 |
95-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| U/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 50 |
2.702 |
2.758 |
2.730 |
2.577 |
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| 25 |
2.211 |
2.219 |
2.215 |
2.062 |
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| 12.5 |
1.566 |
1.596 |
1.581 |
1.428 |
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| 6.25 |
0.984 |
0.997 |
0.991 |
0.838 |
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| 3.12 |
0.562 |
0.581 |
0.572 |
0.419 |
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| 1.56 |
0.439 |
0.407 |
0.423 |
0.270 |
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| 0.78 |
0.291 |
0.281 |
0.286 |
0.133 |
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| 0 |
0.151 |
0.154 |
0.153 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
