| Target Name |
resistin |
| Alternative Names |
Adipose tissue specific secretory factor ELISA Kit; Adipose tissue-specific secretory factor ELISA Kit; ADSF ELISA Kit; C/EBP epsilon regulated myeloid specific secreted cysteine rich protein ELISA Kit; C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1 ELISA Kit; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein ELISA Kit; CG5403 ELISA Kit; Cysteine rich secreted protein A12 alpha like 2 ELISA Kit; Cysteine rich secreted protein FIZZ3 ELISA Kit; Cysteine-rich secreted protein A12-alpha-like 2 ELISA Kit; Cysteine-rich secreted protein FIZZ3 ELISA Kit; dri ELISA Kit; FIZZ 3 ELISA Kit; FIZZ3 ELISA Kit; Found in inflammatory zone 3 ELISA Kit; HXCP 1 ELISA Kit; HXCP1 ELISA Kit; MGC126603 ELISA Kit; MGC126609 ELISA Kit; PRO1199 ELISA Kit; Resistin ELISA Kit; Resistin delta2 ELISA Kit; RETN 1 ELISA Kit; RETN ELISA Kit; RETN_HUMAN ELISA Kit; RETN1 ELISA Kit; RSTN ELISA Kit; UNQ407 ELISA Kit; XCP 1 ELISA Kit; XCP1 ELISA Kit |
| Abbreviation |
RETN |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.312 ng/mL-20 ng/mL |
| Sensitivity |
0.078 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human resistin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
97 |
|
| Range % |
92-103 |
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| 1:2 |
Average % |
94 |
|
| Range % |
91-98 |
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| 1:4 |
Average % |
102 |
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| Range % |
98-106 |
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| 1:8 |
Average % |
86 |
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| Range % |
80-92 |
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| Recovery |
| The recovery of human resistin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
97 |
90-104 |
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| EDTA plasma (n=4) |
92 |
88-97 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 20 |
2.285 |
2.330 |
2.308 |
2.204 |
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| 10 |
1.350 |
1.427 |
1.389 |
1.285 |
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| 5 |
0.701 |
0.733 |
0.717 |
0.613 |
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| 2.5 |
0.400 |
0.424 |
0.412 |
0.308 |
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| 1.25 |
0.256 |
0.268 |
0.262 |
0.158 |
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| 0.625 |
0.178 |
0.169 |
0.174 |
0.070 |
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| 0.312 |
0.132 |
0.129 |
0.131 |
0.027 |
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| 0 |
0.105 |
0.102 |
0.104 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
