Human secretory leukocyte protease inhibitor,SLPI ELISA Kit
Product Details
| Target Name |
secretory leukocyte peptidase inhibitor |
| Alternative Names |
ALK 1 ELISA Kit; ALK1 ELISA Kit; ALP ELISA Kit; Antileukoprotease ELISA Kit; Antileukoproteinase 1 ELISA Kit; Antileukoproteinase ELISA Kit; Antileukoproteinase1 ELISA Kit; BLPI ELISA Kit; Human seminal protease inhibitor ELISA Kit; HUSI 1 ELISA Kit; HUSI ELISA Kit; HUSI I ELISA Kit; HUSI-1 ELISA Kit; MPI ELISA Kit; Mucus proteinase inhibitor ELISA Kit; Protease inhibitor WAP4 ELISA Kit; Secretory leukocyte peptidase inhibitor ELISA Kit; Secretory leukocyte protease inhibitor ELISA Kit; Seminal proteinase inhibitor ELISA Kit; SLPI ELISA Kit; SLPI_HUMAN ELISA Kit; WAP four disulfide core domain protein 4 ELISA Kit; WAP four-disulfide core domain protein 4 ELISA Kit; WAP4 ELISA Kit; WFDC4 ELISA Kit |
| Abbreviation |
SLPI |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.81 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human SLPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
96 |
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| Range % |
91-102 |
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| 1:200 |
Average % |
92 |
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| Range % |
85-98 |
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| 1:400 |
Average % |
89 |
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| Range % |
83-94 |
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| 1:800 |
Average % |
91 |
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| Range % |
87-96 |
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| Recovery |
| The recovery of human SLPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
93-102 |
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| EDTA plasma (n=4) |
86 |
81-92 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.637 |
2.531 |
2.584 |
2.490 |
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| 1000 |
2.124 |
2.205 |
2.165 |
2.071 |
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| 500 |
1.454 |
1.557 |
1.506 |
1.412 |
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| 250 |
0.936 |
0.923 |
0.930 |
0.836 |
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| 125 |
0.448 |
0.478 |
0.463 |
0.369 |
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| 62.5 |
0.283 |
0.274 |
0.279 |
0.185 |
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| 31.25 |
0.156 |
0.163 |
0.160 |
0.066 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
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