Human Serglycin(SRGN) ELISA kit
Product Details
| Target Name |
serglycin |
| Alternative Names |
Chondroitin sulfate proteoglycan core protein ELISA Kit; Cytolytic granule proteoglycan core protein ELISA Kit; FLJ12930 ELISA Kit; gp600 ELISA Kit; Hematopoetic proteoglycan core protein ELISA Kit; Mastocytoma proteoglycan core protein ELISA Kit; MGC9289 ELISA Kit; OTTHUMP00000019716 ELISA Kit; P.PG ELISA Kit; PG19 core protein ELISA Kit; Pgsg ELISA Kit; Platelet proteoglycan core protein ELISA Kit; platelet proteoglycan protein core ELISA Kit; PLATELET PROTEOGLYCAN PROTEIN CORE; PPG ELISA Kit; PPG ELISA Kit; PRG ELISA Kit; PRG1 ELISA Kit; PROTEOGLYCAN 1 ELISA Kit; proteoglycan 1; secretory granule ELISA Kit; Proteoglycan 10K core protein ELISA Kit; Proteoglycan peptide core protein ELISA Kit; PROTEOGLYCAN PROTEIN CORE FOR MAST CELL SECRETORY GRANULE ELISA Kit; secretory granule proteoglycan 1 ELISA Kit; Secretory granule proteoglycan core peptide ELISA Kit; Secretory granule proteoglycan core protein ELISA Kit; Serglycin ELISA Kit; serglycin proteoglycan ELISA Kit; Sgc ELISA Kit; Srgn ELISA Kit; SRGN_HUMAN ELISA Kit |
| Abbreviation |
SRGN |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.312 ng/mL-20 ng/mL |
| Sensitivity |
0.078 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cell Biology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
|
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
|
| Inter-assay Precision (Precision between assays): CV% |
|
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
|
| |
|
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human SRGN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
|
| |
Sample |
Serum(n=4) |
|
|
| 1:1 |
Average % |
107 |
|
|
| Range % |
103-112 |
|
|
| 1:2 |
Average % |
97 |
|
|
| Range % |
93-102 |
|
|
| 1:4 |
Average % |
94 |
|
|
| Range % |
88-98 |
|
|
| 1:8 |
Average % |
96 |
|
|
| Range % |
92-99 |
|
|
|
| Recovery |
| The recovery of human SRGN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| |
| Sample Type |
Average % Recovery |
Range |
|
|
| Serum (n=5) |
93 |
88-97 |
|
|
| EDTA plasma (n=4) |
87 |
82-91 |
|
|
| |
|
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
|
| 20 |
2.612 |
2.535 |
2.574 |
2.414 |
|
|
| 10 |
2.265 |
2.171 |
2.218 |
2.058 |
|
|
| 5 |
1.667 |
1.632 |
1.650 |
1.490 |
|
|
| 2.5 |
1.078 |
1.064 |
1.071 |
0.911 |
|
|
| 1.25 |
0.675 |
0.669 |
0.672 |
0.512 |
|
|
| 0.625 |
0.443 |
0.438 |
0.441 |
0.281 |
|
|
| 0.312 |
0.328 |
0.323 |
0.326 |
0.166 |
|
|
| 0 |
0.161 |
0.159 |
0.160 |
|
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
