Human Serine protease HTRA1(HTRA1) ELISA kit
Product Details
| Target Name |
HtrA serine peptidase 1 |
| Alternative Names |
ARMD7 ELISA Kit; CARASIL ELISA Kit; High-temperature requirement A serine peptidase 1 ELISA Kit; HtrA ELISA Kit; HtrA serine peptidase 1 ELISA Kit; HTRA1 ELISA Kit; HTRA1_HUMAN ELISA Kit; IGFBP5 protease ELISA Kit; L56 ELISA Kit; ORF480 ELISA Kit; Protease serine 11 (IGF binding) ELISA Kit; protease serine 11 ELISA Kit; PRSS11 ELISA Kit; Serine protease 11 ELISA Kit; Serine protease HTRA1 ELISA Kit; Serine protease HTRA1 precursor ELISA Kit |
| Abbreviation |
HTRA1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates |
| Detection Range |
1.56 ng/mL-100 ng/mL |
| Sensitivity |
0.39 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human HTRA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
84-96 |
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| 1:2 |
Average % |
96 |
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| Range % |
92-103 |
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| 1:4 |
Average % |
98 |
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| Range % |
94-102 |
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| 1:8 |
Average % |
86 |
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| Range % |
82-93 |
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| Recovery |
| The recovery of human HTRA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
99 |
84-105 |
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| EDTA plasma (n=4) |
98 |
92-104 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 100 |
2.357 |
2.405 |
2.381 |
2.282 |
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| 50 |
2.112 |
2.212 |
2.162 |
2.063 |
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| 25 |
1.842 |
1.700 |
1.771 |
1.672 |
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| 12.5 |
1.275 |
1.295 |
1.285 |
1.186 |
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| 6.25 |
0.823 |
0.888 |
0.856 |
0.757 |
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| 3.12 |
0.481 |
0.503 |
0.492 |
0.393 |
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| 1.56 |
0.258 |
0.263 |
0.261 |
0.162 |
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| 0 |
0.103 |
0.095 |
0.099 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
