| Target Name |
slit homolog 2 (Drosophila) |
| Alternative Names |
Drad 1 ELISA Kit; E030015M03Rik ELISA Kit; E130320P19Rik ELISA Kit; FLJ14420 ELISA Kit; OTTHUMP00000158695 ELISA Kit; OTTHUMP00000217852 ELISA Kit; OTTHUMP00000217853 ELISA Kit; OTTHUMP00000217854 ELISA Kit; Slil 3 ELISA Kit; Slil3 ELISA Kit; Slit 2 ELISA Kit; Slit homolog 2 (Drosophila) ELISA Kit; Slit homolog 2 ELISA Kit; Slit homolog 2 protein ELISA Kit; Slit homolog 2 protein C-product ELISA Kit; Slit-2 ELISA Kit; Slit2 ELISA Kit; SLIT2_HUMAN ELISA Kit |
| Abbreviation |
SLIT2 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.78 ng/mL-50 ng/mL |
| Sensitivity |
0.195 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Neuroscience |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human Slit2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
92 |
|
| Range % |
88-95 |
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| 1:2 |
Average % |
94 |
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| Range % |
90-98 |
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| 1:4 |
Average % |
93 |
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| Range % |
88-95 |
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| 1:8 |
Average % |
98 |
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| Range % |
94-103 |
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| Recovery |
| The recovery of human Slit2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
91-96 |
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| EDTA plasma (n=4) |
95 |
91-97 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 50 |
2.957 |
2.912 |
2.935 |
2.770 |
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| 25 |
2.313 |
2.284 |
2.299 |
2.134 |
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| 12.5 |
1.472 |
1.388 |
1.430 |
1.265 |
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| 6.25 |
0.797 |
0.804 |
0.801 |
0.636 |
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| 3.12 |
0.400 |
0.406 |
0.403 |
0.238 |
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| 1.56 |
0.285 |
0.302 |
0.294 |
0.129 |
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| 0.78 |
0.254 |
0.244 |
0.249 |
0.084 |
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| 0 |
0.167 |
0.163 |
0.165 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
