The Human soluble CD163 (sCD163) ELISA kit is designed for detection and quantification of human sCD163 in serum, plasma, or tissue homogenates. It has been validated with high sensitivity, strong specificity, good linearity, precision less than 10%, high recovery, and consistency across batches. Refer to the product instructions for more details.
The measurement of this assay is based on the Sandwich-ELISA technique. The samples and biotin-labeled CD163 antibody are successively pipetted into the wells pre-coated with anti-human CD163 antibody. The CD163 is sandwiched between the two antibodies, forming Ag-Ab-Ag immune complex. HRP-avidin is subsequently added to the wells. After thorough washing, the substrate TMB is added for color development. TMB is converted to blue by HRP and yellow by the stop solution. The intensity of the color is positively correlated with the CD163 content in the samples.
CD163, a scavenger receptor, is expressed solely in cells of monocytic origin with a high expression in M2 macrophages where it exerts an established role in scavenging hemoglobin (Hb) and haptoglobin (Hp)-Hb complexes released into the plasma. It possesses anti-inflammatory and immunoregulatory properties. Upon ectodomain shedding induced by an inflammatory stimulus, CD163 becomes the soluble form sCD163 in plasma. Blood sCD163 levels are linked to acute and chronic inflammatory processes in autoimmune disorders of connective tissue, fat metabolism, and cardiovascular diseases. And sCD163 concentration in blood can be the marker for the assessment of cancer prognosis.
