Product Details
Description
This ELISA kit is used for quantitative detection of a human soluble cluster of differentiation 14 (sCD14), establishing normal detection range and validation of antibody array results. CD14 is a pattern-recognition receptor and exists in two different forms: GPI-anchored membrane CD14 (mCD14) and liver-derived serum soluble CD14. sCD14 is an acute-phase protein upregulated significantly during sepsis but is also found in normal serum at microgram concentrations. CD14 plays an important role in the phagocytic clearance of apoptotic cells and the reactivation and immune recognition of microbial cell wall components from Gran-positive and Gram-negative bacteria. sCD14 can transfer the LPS to the TLR4/MD-2 complex, eliciting a cascade of inflammatory signaling and TLR4 endocytosis. Based on the Sandwich-ELISA principle and enzyme-substrate chromogenic reaction property, the users can measure the content of human CD14 in serum, plasma, cell culture supernates, tissue homogenates, or urine....Read more
Target Name
CD14 molecule
Alternative Names
CD 14 ELISA Kit; CD_antigen=CD14 ELISA Kit; CD14 ELISA Kit; CD14 antigen ELISA Kit; CD14 molecule ELISA Kit; CD14_HUMAN ELISA Kit; LPS-R ELISA Kit; Mo2 ELISA Kit; Monocyte differentiation antigen CD14 ELISA Kit; Monocyte differentiation antigen CD14 urinary form ELISA Kit; Monocyte differentiation antigen CD14; membrane-bound form ELISA Kit; Myeloid cell specific leucine rich glycoprotein ELISA Kit; Myeloid cell-specific leucine-rich glycoprotein ELISA Kit
Abbreviation
CD14
Uniprot No.
P08571
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates, urine
Detection Range
0.234 ng/mL-15 ng/mL
Sensitivity
0.229 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Storage
Store at 2-8°C. Please refer to protocol.