Human Soluble Cluster of Differentiation 80 (sCD80) ELISA Kit
Product Details
| Target Name |
CD80 molecule |
| Alternative Names |
Activation B7-1 antigen ELISA Kit; B lymphocyte activation antigen B7 ELISA Kit; B7 ELISA Kit; B7-1 ELISA Kit; B7-1 antigen ELISA Kit; B7.1 ELISA Kit; BB1 ELISA Kit; CD28 antigen ligand 1 ELISA Kit; CD28LG ELISA Kit; CD28LG1 ELISA Kit; CD80 ELISA Kit; CD80 antigen (CD28 antigen ligand 1; B7-1 antigen) ELISA Kit; CD80 antigen ELISA Kit; CD80 molecule ELISA Kit; CD80_HUMAN ELISA Kit; Costimulatory factor CD80 ELISA Kit; costimulatory molecule variant IgV-CD80 ELISA Kit; CTLA-4 counter-receptor B7.1 ELISA Kit; LAB7 ELISA Kit; T-lymphocyte activation antigen CD80 ELISA Kit |
| Abbreviation |
CD80 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, cell culture supernates |
| Detection Range |
125 pg/mL-8000 pg/mL |
| Sensitivity |
31.25 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human sCD80 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
103 |
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| Range % |
99-107 |
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| 1:2 |
Average % |
87 |
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| Range % |
82-91 |
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| 1:4 |
Average % |
96 |
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| Range % |
92-100 |
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| 1:8 |
Average % |
90 |
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| Range % |
85-95 |
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| Recovery |
| The recovery of human sCD80 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
85 |
80-89 |
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| EDTA plasma (n=4) |
94 |
90-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 8000 |
2.618 |
2.485 |
2.552 |
2.435 |
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| 4000 |
1.805 |
1.693 |
1.749 |
1.632 |
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| 2000 |
1.004 |
1.047 |
1.026 |
0.909 |
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| 1000 |
0.547 |
0.562 |
0.555 |
0.438 |
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| 500 |
0.351 |
0.334 |
0.343 |
0.226 |
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| 250 |
0.230 |
0.219 |
0.225 |
0.108 |
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| 125 |
0.162 |
0.171 |
0.167 |
0.050 |
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| 0 |
0.113 |
0.120 |
0.117 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
