Human soluble Cluster of differentiation 86,sCD86 ELISA Kit
Product Details
| Target Name |
CD86 molecule |
| Alternative Names |
Activation B7-2 antigen 3 ELISA Kit; Activation B7-2 antigen ELISA Kit; B-lymphocyte activation antigen B7-2 2 ELISA Kit; B-lymphocyte activation antigen B7-2 ELISA Kit; B7 2 ELISA Kit; B7 ELISA Kit; B7-2 ELISA Kit; B7.2 ELISA Kit; B70 ELISA Kit; B72 ELISA Kit; B72 antigen ELISA Kit; BU63 ELISA Kit; CD28 antigen ligand 2 2 ELISA Kit; CD28 antigen ligand 2 ELISA Kit; Cd28l2 ELISA Kit; CD28LG2 ELISA Kit; CD86 ELISA Kit; CD86 antigen (CD28 antigen ligand 2 B7 2 antigen) ELISA Kit; CD86 antigen (CD28 antigen ligand 2; B7-2 antigen) 1; 2 ELISA Kit; CD86 antigen (CD28 antigen ligand 2; B7-2 antigen) ELISA Kit; CD86 antigen ELISA Kit; CD86 molecule ELISA Kit; CD86_HUMAN ELISA Kit; CLS1 ELISA Kit; CTLA-4 counter-receptor B7.2 2 ELISA Kit; CTLA-4 counter-receptor B7.2 2; 3 ELISA Kit; CTLA-4 counter-receptor B7.2 ELISA Kit; Early T-cell costimulatory molecule 1 ELISA Kit; ETC-1 ELISA Kit; FUN 1 ELISA Kit; FUN-1 ELISA Kit; FUN1 ELISA Kit; LAB72 ELISA Kit; Ly-58 ELISA Kit; Ly58 ELISA Kit; MB7 ELISA Kit; MB7-2 ELISA Kit; Membrane glycoprotein ELISA Kit; MGC34413 ELISA Kit; T lymphocyte activation antigen CD86 precursor ELISA Kit; T-lymphocyte activation antigen CD86 ELISA Kit; TS/A-2 ELISA Kit |
| Abbreviation |
CD86 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.312 ng/mL-20 ng/mL |
| Sensitivity |
0.078 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human sCD86 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
91-95 |
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| 1:2 |
Average % |
96 |
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| Range % |
91-101 |
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| 1:4 |
Average % |
99 |
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| Range % |
92-106 |
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| 1:8 |
Average % |
86 |
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| Range % |
82-90 |
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| Recovery |
| The recovery of human sCD86 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
89-95 |
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| EDTA plasma (n=4) |
103 |
100-106 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.500 |
2.659 |
2.580 |
2.425 |
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| 10 |
2.167 |
2.035 |
2.101 |
1.946 |
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| 5 |
1.423 |
1.544 |
1.484 |
1.329 |
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| 2.5 |
0.888 |
0.852 |
0.870 |
0.715 |
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| 1.25 |
0.622 |
0.631 |
0.627 |
0.472 |
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| 0.625 |
0.414 |
0.437 |
0.426 |
0.271 |
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| 0.312 |
0.268 |
0.281 |
0.275 |
0.120 |
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| 0 |
0.149 |
0.161 |
0.155 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
