Human soluble endothelial protein C receptor,sEPCR ELISA Kit
Product Details
| Description |
This ELISA kit is used for quantitative detection of human endothelial protein C receptor (sEPCR), establishing normal detection range and validation of antibody array results. EPCR is a type I transmembrane glycoprotein expressed mainly on the luminal surface of endothelial cells. It is one of the most important components of the protein C (PC) anticoagulant pathway, classically known as the anticoagulant system, which plays an important role in controlling thrombosis and in limiting the inflammatory response. sEPCR, a soluble form of EPCR in plasma, is shed from the EPCR membrane upon cleavage by metalloproteases. sEPCR exerts procoagulant and proinflammatory effects. It binds to PC or activated PC (APC), leading to PC's unavailability for activation by thrombin-thrombomodulin (TM) complex and the inhibition of APC anticoagulant activity. Additionally, sEPCR regulates inflammation by binding to activated neutrophils and factor VIIa, reducing the ability of FVIIa to activate FX. Based... Read more |
| Target Name |
protein C receptor, endothelial (EPCR) |
| Alternative Names |
Activated protein C receptor ELISA Kit; APC receptor ELISA Kit; APCR ELISA Kit; bA42O4.2 ELISA Kit; CCCA ELISA Kit; CCD41 ELISA Kit; CD201 ELISA Kit; CD201 antigen ELISA Kit; cell cycle; centrosome-associated protein ELISA Kit; Cell cycle; centrosome-associated protein ELISA Kit; centrocyclin ELISA Kit; Endothelial cell protein C receptor ELISA Kit; Endothelial protein C receptor ELISA Kit; EPCR ELISA Kit; EPCR_HUMAN ELISA Kit; MGC23024 ELISA Kit; PROCR ELISA Kit; Protein C receptor; endothelial ELISA Kit |
| Abbreviation |
PROCR |
| Uniprot No. |
Q9UNN8 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
7.8 ng/mL-500 ng/mL |
| Sensitivity |
1.95 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human sEPCR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
89-95 |
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| 1:2 |
Average % |
102 |
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| Range % |
99-105 |
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| 1:4 |
Average % |
93 |
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| Range % |
85-95 |
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| 1:8 |
Average % |
99 |
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| Range % |
94-103 |
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| Recovery |
| The recovery of human sEPCR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
90-97 |
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| EDTA plasma (n=4) |
95 |
91-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 500 |
2.830 |
2.812 |
2.821 |
2.682 |
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| 250 |
2.212 |
2.200 |
2.206 |
2.067 |
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| 125 |
1.412 |
1.436 |
1.424 |
1.285 |
|
| 62.5 |
0.752 |
0.712 |
0.732 |
0.593 |
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| 31.2 |
0.366 |
0.384 |
0.375 |
0.236 |
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| 15.6 |
0.279 |
0.261 |
0.270 |
0.131 |
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| 7.8 |
0.215 |
0.200 |
0.208 |
0.069 |
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| 0 |
0.140 |
0.138 |
0.139 |
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| Materials provided |
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human EPCR antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled EPCR antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
