Human soluble OX40L (sOX40L) ELISA kit
Product Details
| Target Name |
tumor necrosis factor (ligand) superfamily, member 4 |
| Alternative Names |
CD 134L ELISA Kit; CD 252 ELISA Kit; CD134 ligand ELISA Kit; CD134L ELISA Kit; CD252 ELISA Kit; CD252 antigen ELISA Kit; glycoprotein 34 kd ELISA Kit; Glycoprotein Gp34 ELISA Kit; GP34 ELISA Kit; OX-40L ELISA Kit; OX40 antigen ligand ELISA Kit; OX40 ligand ELISA Kit; OX40L ELISA Kit; TAX transcriptionally-activated glycoprotein 1 ELISA Kit; TNFL4_HUMAN ELISA Kit; Tnfsf4 ELISA Kit; Tumor necrosis factor (ligand) superfamily member 4 ELISA Kit; Tumor necrosis factor ligand superfamily member 4 ELISA Kit; TXGP1 ELISA Kit |
| Abbreviation |
TNFSF4 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human sOX40L in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
106 |
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| Range % |
100-113 |
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| 1:2 |
Average % |
100 |
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| Range % |
95-105 |
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| 1:4 |
Average % |
89 |
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| Range % |
82-98 |
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| 1:8 |
Average % |
97 |
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| Range % |
92-102 |
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| Recovery |
| The recovery of human sOX40L spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
93 |
87-98 |
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| EDTA plasma (n=4) |
94 |
90-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.554 |
2.603 |
2.579 |
2.450 |
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| 5 |
1.975 |
2.065 |
2.020 |
1.891 |
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| 2.5 |
1.305 |
1.402 |
1.354 |
1.225 |
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| 1.25 |
0.744 |
0.714 |
0.729 |
0.600 |
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| 0.625 |
0.476 |
0.498 |
0.487 |
0.358 |
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| 0.312 |
0.351 |
0.326 |
0.339 |
0.210 |
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| 0.156 |
0.227 |
0.226 |
0.227 |
0.098 |
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| 0 |
0.132 |
0.126 |
0.129 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
