Human Soluble protein-100B,S-100B ELISA Kit
Product Details
| Description |
The product CSB-E08065h is a ready-to-use microwell, strip plate ELISA kit for quantitatively analyzing the presence of the Soluble protein-100B (S-100B) in different biological samples, including serum, plasma, tissue homogenates, and cerebrospinal fluid (CSF). This S100B ELISA kit specifically recognizes the human S-100B in the sample. It adopts the Sandwich ELISA technique in conjunction with antibody-antigen specific binding and an enzyme colorimetric reaction to indicate the presence of human S-100B and quantitatively measure the concentration of S-100B in the samples. This kit has been quality controlled in many performances, including sensitivity, specificity, recovery, precision, linearity, and lot-to-lot consistency. S100B is a cytosolic calcium-binding protein concentrated mainly in the glial cells of the central nervous system (CNS). It is primarily released from the astroglial cells into blood and urine after the brain undergoing hypoxic-ischemic damage. Serum S100B concent... Read more |
| Target Name |
S100 calcium binding protein B |
| Alternative Names |
NEF ELISA Kit; Protein S100 B ELISA Kit; Protein S100-B ELISA Kit; S 100 calcium binding protein beta chain ELISA Kit; S 100 protein beta chain ELISA Kit; S-100 protein beta chain ELISA Kit; S-100 protein subunit beta ELISA Kit; S100 ELISA Kit; S100 calcium binding protein beta (neural) ELISA Kit; S100 calcium-binding protein B ELISA Kit; S100 protein beta chain ELISA Kit; S100B ELISA Kit; S100B_HUMAN ELISA Kit; S100beta ELISA Kit |
| Abbreviation |
S100B |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cerebrospinal fluid (CSF) |
| Detection Range |
78 pg/mL-5000 pg/mL |
| Sensitivity |
19.5 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human S-100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
84 |
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| Range % |
80-95 |
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| 1:2 |
Average % |
89 |
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| Range % |
85-94 |
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| 1:4 |
Average % |
95 |
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| Range % |
92-100 |
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| 1:8 |
Average % |
90 |
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| Range % |
85-98 |
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| Recovery |
| The recovery of human S-100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
85-99 |
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| EDTA plasma (n=4) |
96 |
92-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 5000 |
2.037 |
1.902 |
1.970 |
1.809 |
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| 2500 |
1.585 |
1.583 |
1.584 |
1.423 |
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| 1250 |
1.184 |
1.156 |
1.170 |
1.009 |
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| 625 |
0.821 |
0.852 |
0.837 |
0.676 |
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| 312 |
0.595 |
0.581 |
0.588 |
0.427 |
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| 156 |
0.372 |
0.380 |
0.376 |
0.215 |
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| 78 |
0.249 |
0.234 |
0.242 |
0.081 |
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| 0 |
0.164 |
0.158 |
0.161 |
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| Materials provided |
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human S100B antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled S100B antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
