Human Soluble Receptor Activator of Nuclear Factor-kB,sRANK ELISA Kit
Product Details
| Target Name |
tumor necrosis factor receptor superfamily, member 11a, NFKB activator |
| Alternative Names |
CD 265 ELISA Kit; CD265 ELISA Kit; FEO ELISA Kit; LOH18CR1 ELISA Kit; Loss of heterozygosity 18 chromosomal region 1 ELISA Kit; mRANK ELISA Kit; ODFR ELISA Kit; OFE ELISA Kit; OPTB7 ELISA Kit; Osteoclast differentiation factor receptor ELISA Kit; OSTS ELISA Kit; Paget disease of bone 2 ELISA Kit; PDB 2 ELISA Kit; PDB2 ELISA Kit; RANK ELISA Kit; Receptor activator of NF KB ELISA Kit; Receptor activator of NF-KB ELISA Kit; receptor activator of nuclear factor kappa B ELISA Kit; TNF receptor superfamily member 11a ELISA Kit; TNFRSF11A ELISA Kit; TNR11_HUMAN ELISA Kit; TRANCER ELISA Kit; Tumor necrosis factor receptor superfamily member 11a NFKB activator ELISA Kit; Tumor necrosis factor receptor superfamily member 11a activator of NFKB ELISA Kit; Tumor necrosis factor receptor superfamily member 11A ELISA Kit |
| Abbreviation |
TNFRSF11A |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
18.75 pg/mL-1200 pg/mL |
| Sensitivity |
4.68 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human sRANK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
86-94 |
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| 1:2 |
Average % |
101 |
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| Range % |
94-104 |
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| 1:4 |
Average % |
91 |
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| Range % |
85-94 |
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| 1:8 |
Average % |
95 |
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| Range % |
90-98 |
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| Recovery |
| The recovery of human sRANK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
84-94 |
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| EDTA plasma (n=4) |
95 |
89-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1200 |
1.981 |
2.012 |
1.997 |
1.898 |
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| 600 |
1.523 |
1.554 |
1.539 |
1.440 |
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| 300 |
0.886 |
0.867 |
0.877 |
0.778 |
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| 150 |
0.425 |
0.445 |
0.435 |
0.336 |
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| 75 |
0.290 |
0.303 |
0.297 |
0.198 |
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| 37.5 |
0.215 |
0.222 |
0.219 |
0.120 |
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| 18.75 |
0.154 |
0.157 |
0.156 |
0.057 |
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| 0 |
0.100 |
0.098 |
0.099 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
