Human soluble tumor necrosis factor-like weak inducer of apoptosis (sTWEAK)ELISA Kit
Product Details
| Target Name |
tumor necrosis factor (ligand) superfamily, member 12 |
| Alternative Names |
APO 3 ligand ELISA Kit; APO 3L ELISA Kit; APO3 ligand ELISA Kit; APO3/DR3 ligand ELISA Kit; APO3L ELISA Kit; DR3LG ELISA Kit; MGC129581 ELISA Kit; MGC20669 ELISA Kit; secreted form ELISA Kit; TNF-related weak inducer of apoptosis ELISA Kit; TNF12_HUMAN ELISA Kit; TNFSF 12 ELISA Kit; Tnfsf12 ELISA Kit; TNFSF12 protein ELISA Kit; Tumor necrosis factor (ligand) superfamily member 12 ELISA Kit; Tumor necrosis factor ligand superfamily member 12 ELISA Kit; Tumor necrosis factor superfamily member 12 ELISA Kit; TWEAK ELISA Kit; UNQ181/PRO207 ELISA Kit |
| Abbreviation |
TNFSF12 |
| Uniprot No. |
O43508 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.312 ng/ml-20 ng/ml |
| Sensitivity |
0.078 ng/ml |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human sTWEAK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
87 |
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| Range % |
80-98 |
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| 1:2 |
Average % |
95 |
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| Range % |
92-104 |
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| 1:4 |
Average % |
94 |
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| Range % |
89-100 |
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| 1:8 |
Average % |
92 |
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| Range % |
85-100 |
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| Recovery |
| The recovery of human sTWEAK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
87 |
82-100 |
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| EDTA plasma (n=4) |
92 |
90-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.501 |
2.495 |
2.498 |
2.336 |
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| 1000 |
1.862 |
1.884 |
1.873 |
1.711 |
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| 500 |
1.174 |
1.172 |
1.173 |
1.011 |
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| 250 |
0.702 |
0.687 |
0.695 |
0.533 |
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| 125 |
0.422 |
0.402 |
0.412 |
0.250 |
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| 62.5 |
0.310 |
0.291 |
0.301 |
0.139 |
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| 31.25 |
0.207 |
0.221 |
0.214 |
0.052 |
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| 0 |
0.159 |
0.165 |
0.162 |
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Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
