Human soluble tumor necrosis factor receptor 2,sTNF-R2 ELISA Kit
Product Details
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| Target Name |
tumor necrosis factor receptor superfamily, member 1B |
| Alternative Names |
CD120b ELISA Kit; p75 ELISA Kit; p75 TNF receptor ELISA Kit; p75TNFR ELISA Kit; p80 TNF alpha receptor ELISA Kit; p80 TNF-alpha receptor ELISA Kit; Soluble TNFR1B variant 1 ELISA Kit; TBP-2 ELISA Kit; TBPII ELISA Kit; TNF R II ELISA Kit; TNF R2 ELISA Kit; TNF R75 ELISA Kit; TNF-R2 ELISA Kit; TNF-RII ELISA Kit; TNFBR ELISA Kit; TNFR-II ELISA Kit; TNFR1B ELISA Kit; TNFR2 ELISA Kit; TNFR80 ELISA Kit; TNFRII ELISA Kit; Tnfrsf1b ELISA Kit; TNR1B_HUMAN ELISA Kit; Tumor necrosis factor beta receptor ELISA Kit; Tumor necrosis factor binding protein 2 ELISA Kit; Tumor necrosis factor receptor 2 ELISA Kit; Tumor necrosis factor receptor superfamily member 1B ELISA Kit; Tumor necrosis factor receptor type II ELISA Kit; Tumor necrosis factor-binding protein 2 ELISA Kit |
| Abbreviation |
TNFRSF1B |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.8 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human sTNF-R2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
101 |
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| Range % |
95-105 |
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| 1:2 |
Average % |
93 |
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| Range % |
87-97 |
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| 1:4 |
Average % |
97 |
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| Range % |
85-101 |
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| 1:8 |
Average % |
94 |
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| Range % |
85-99 |
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| Recovery |
| The recovery of human sTNF-R2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
100 |
94-104 |
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| EDTA plasma (n=4) |
101 |
92-104 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.170 |
2.099 |
2.135 |
2.032 |
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| 1000 |
1.721 |
1.788 |
1.755 |
1.652 |
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| 500 |
1.272 |
1.243 |
1.258 |
1.155 |
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| 250 |
0.776 |
0.807 |
0.792 |
0.689 |
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| 125 |
0.487 |
0.501 |
0.494 |
0.391 |
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| 62.5 |
0.306 |
0.322 |
0.314 |
0.211 |
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| 31.25 |
0.209 |
0.213 |
0.211 |
0.108 |
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| 0 |
0.102 |
0.104 |
0.103 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
