Human Sortilin(SORT1) ELISA kit
Product Details
| Target Name |
sortilin 1 |
| Alternative Names |
100 kDa NT receptor ELISA Kit; Glycoprotein 95 ELISA Kit; Gp 95 ELISA Kit; Gp95 ELISA Kit; LDLCQ6 ELISA Kit; Neurotensin receptor 3 ELISA Kit; NT 3 ELISA Kit; NT3 ELISA Kit; NTR 3 ELISA Kit; NTR3 ELISA Kit; OTTHUMP00000013784 ELISA Kit; SORT 1 ELISA Kit; SORT_HUMAN ELISA Kit; SORT1 (gene name) ELISA Kit; Sort1 ELISA Kit; Sortilin 1 ELISA Kit; Sortilin ELISA Kit |
| Abbreviation |
SORT1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
46.88 pg/mL-3000 pg/mL |
| Sensitivity |
11.72 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human sortilin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
86-93 |
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| 1:2 |
Average % |
104 |
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| Range % |
100-107 |
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| 1:4 |
Average % |
94 |
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| Range % |
88-97 |
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| 1:8 |
Average % |
99 |
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| Range % |
93-103 |
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| Recovery |
| The recovery of human sortilin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
90-97 |
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| EDTA plasma (n=4) |
97 |
93-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 3000 |
2.349 |
2.333 |
2.341 |
2.184 |
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| 1500 |
1.864 |
1.776 |
1.820 |
1.663 |
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| 750 |
1.312 |
1.302 |
1.307 |
1.150 |
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| 375 |
0.943 |
0.922 |
0.933 |
0.776 |
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| 187.5 |
0.654 |
0.634 |
0.644 |
0.487 |
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| 93.75 |
0.463 |
0.456 |
0.460 |
0.303 |
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| 46.88 |
0.317 |
0.304 |
0.311 |
0.154 |
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| 0 |
0.158 |
0.156 |
0.157 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
