Human SPARC(SPARC) ELISA kit
Product Details
| Target Name |
secreted protein, acidic, cysteine-rich (osteonectin) |
| Alternative Names |
AA517111 ELISA Kit; Basement membrane protein 40 ELISA Kit; Basement-membrane protein 40 ELISA Kit; BM 40 ELISA Kit; BM-40 ELISA Kit; BM40 ELISA Kit; Cysteine rich protein ELISA Kit; hm:zeh0062 ELISA Kit; MGC128090 ELISA Kit; OI17 ELISA Kit; ON ELISA Kit; Osteonectin ELISA Kit; Secreted acidic cystein rich glycoprotein ELISA Kit; Secreted protein acidic and cysteine rich ELISA Kit; Secreted protein acidic and rich in cysteine ELISA Kit; Secreted protein acidic cysteine rich (osteonectin) ELISA Kit; Secreted protein acidic cysteine rich ELISA Kit; SPARC ELISA Kit; SPRC ELISA Kit; SPRC_HUMAN ELISA Kit |
| Abbreviation |
SPARC |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, urine |
| Detection Range |
62.5 ng/mL-4000 ng/mL |
| Sensitivity |
15.6 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human SPARC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
84 |
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| Range % |
81-89 |
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| 1:2 |
Average % |
93 |
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| Range % |
89-97 |
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| 1:4 |
Average % |
98 |
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| Range % |
94-102 |
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| 1:8 |
Average % |
100 |
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| Range % |
95-105 |
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| Recovery |
| The recovery of human SPARC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
87-93 |
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| EDTA plasma (n=4) |
101 |
97-104 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 4000 |
2.120 |
2.301 |
2.211 |
2.049 |
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| 2000 |
1.667 |
1.642 |
1.655 |
1.493 |
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| 1000 |
1.173 |
1.163 |
1.168 |
1.006 |
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| 500 |
0.759 |
0.787 |
0.773 |
0.611 |
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| 250 |
0.453 |
0.469 |
0.461 |
0.299 |
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| 125 |
0.307 |
0.301 |
0.304 |
0.142 |
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| 62.5 |
0.254 |
0.259 |
0.257 |
0.095 |
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| 0 |
0.163 |
0.160 |
0.162 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
