Human Stem Cell Factor Receptor,SCFR ELISA kit
Product Details
| Target Name |
Stem Cell Factor Receptor,SCFR |
| Alternative Names |
C Kit ELISA Kit; c-Kit ELISA Kit; c-Kit Ligand ELISA Kit; CD117 ELISA Kit; Kit ELISA Kit; Kit Ligand ELISA Kit; KIT oncogene ELISA Kit; KIT proto oncogene receptor tyrosine kinase ELISA Kit; KIT_HUMAN ELISA Kit; Mast cell growth factor receptor ELISA Kit; Mast/stem cell growth factor receptor Kit ELISA Kit; MGF ELISA Kit; p145 c-kit ELISA Kit; PBT ELISA Kit; Piebald trait protein ELISA Kit; Proto oncogene c Kit ELISA Kit; Proto oncogene tyrosine protein kinase Kit ELISA Kit; Proto-oncogene c-Kit ELISA Kit; SCF Receptor ELISA Kit; SCFR ELISA Kit; soluble KIT variant 1 ELISA Kit; Steel Factor Receptor ELISA Kit; Stem cell factor receptor ELISA Kit; tyrosine protein kinase Kit ELISA Kit; Tyrosine-protein kinase Kit ELISA Kit; v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog ELISA Kit; v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein ELISA Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog ELISA Kit |
| Abbreviation |
SCFR |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
1.56 ng/mL-100 ng/mL |
| Sensitivity |
0.39 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human SCFR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
85-103 |
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| 1:2 |
Average % |
89 |
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| Range % |
85-99 |
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| 1:4 |
Average % |
93 |
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| Range % |
86-98 |
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| 1:8 |
Average % |
92 |
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| Range % |
83-100 |
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| Recovery |
| The recovery of human SCFR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
86-101 |
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| EDTA plasma (n=4) |
97 |
91-105 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 100 |
1.891 |
1.916 |
1.904 |
1.741 |
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| 50 |
1.349 |
1.397 |
1.373 |
1.210 |
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| 25 |
0.954 |
0.992 |
0.973 |
0.810 |
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| 12.5 |
0.632 |
0.654 |
0.643 |
0.480 |
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| 6.25 |
0.435 |
0.426 |
0.431 |
0.268 |
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| 3.12 |
0.318 |
0.307 |
0.313 |
0.150 |
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| 1.56 |
0.249 |
0.254 |
0.252 |
0.089 |
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| 0 |
0.161 |
0.165 |
0.163 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
