Human Sulfotransferase 1A1(SULT1A1) ELISA kit
Product Details
| Target Name |
sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1 |
| Alternative Names |
Aryl sulfotransferase 1 ELISA Kit; HAST1/HAST2 ELISA Kit; Human aryl sulfotransferase mRNA complete cds ELISA Kit; MGC131921 ELISA Kit; MGC5163 ELISA Kit; OTTHUMP00000162568 ELISA Kit; OTTHUMP00000162569 ELISA Kit; P PST 1 ELISA Kit; P PST ELISA Kit; P-PST 1 ELISA Kit; Phenol sulfating phenol sulfotransferase 1 ELISA Kit; Phenol sulfotransferase 1 ELISA Kit; Phenol-sulfating phenol sulfotransferase 1 ELISA Kit; PST ELISA Kit; ST1A1 ELISA Kit; ST1A1_HUMAN ELISA Kit; ST1A3 ELISA Kit; STP ELISA Kit; STP1 ELISA Kit; Sulfotransferase 1A1 ELISA Kit; Sulfotransferase family 1A phenol preferring member 1 ELISA Kit; Sulfotransferase family cytosolic 1A phenol preferring member 1 ELISA Kit; Sulfotransferase phenol preferring 1 ELISA Kit; SULT1A1 ELISA Kit; Thermostable phenol sulfotransferase ELISA Kit; Thermostable phenol sulfotransferase1 ELISA Kit; Ts-PST ELISA Kit; TSPST1 ELISA Kit |
| Abbreviation |
SULT1A1 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
25 pg/mL-1600 pg/mL |
| Sensitivity |
6.25 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human SULT1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
80-89 |
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| 1:2 |
Average % |
103 |
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| Range % |
98-107 |
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| 1:4 |
Average % |
88 |
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| Range % |
84-92 |
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| 1:8 |
Average % |
98 |
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| Range % |
94-102 |
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| Recovery |
| The recovery of human SULT1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
91 |
85-95 |
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| EDTA plasma (n=4) |
95 |
90-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1600 |
2.078 |
2.148 |
2.113 |
1.918 |
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| 800 |
1.512 |
1.468 |
1.490 |
1.295 |
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| 400 |
1.004 |
1.020 |
1.012 |
0.817 |
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| 200 |
0.733 |
0.689 |
0.711 |
0.516 |
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| 100 |
0.496 |
0.511 |
0.504 |
0.309 |
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| 50 |
0.381 |
0.374 |
0.378 |
0.183 |
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| 25 |
0.275 |
0.286 |
0.281 |
0.086 |
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| 0 |
0.197 |
0.192 |
0.195 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
5-7 working days |
