Human T-cell immunoglobulin and mucin domain-containing protein 4(TIMD4) ELISA kit
Product Details
| Target Name |
T-cell immunoglobulin and mucin domain containing 4 |
| Alternative Names |
SMUCKLER ELISA Kit; Spleen; mucin-containing; knockout of lymphotoxin protein ELISA Kit; T cell immunoglobulin and mucin domain containing protein 4 ELISA Kit; T-cell immunoglobulin and mucin domain containing 4 ELISA Kit; T-cell immunoglobulin and mucin domain containing molecule ELISA Kit; T-cell immunoglobulin and mucin domain-containing protein 4 ELISA Kit; T-cell immunoglobulin and mucin domains-containing protein 4 ELISA Kit; T-cell membrane protein 4 ELISA Kit; TIM-4 ELISA Kit; Tim4 ELISA Kit; TIMD 4 ELISA Kit; TIMD-4 ELISA Kit; Timd4 ELISA Kit; TIMD4_HUMAN ELISA Kit |
| Abbreviation |
TIMD4 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human TIMD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
98 |
|
| Range % |
93-102 |
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| 1:2 |
Average % |
99 |
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| Range % |
92-102 |
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| 1:4 |
Average % |
88 |
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| Range % |
82-91 |
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| 1:8 |
Average % |
95 |
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| Range % |
91-99 |
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| Recovery |
| The recovery of human TIMD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
91 |
82-94 |
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| EDTA plasma (n=4) |
100 |
94-104 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.517 |
2.476 |
2.497 |
2.351 |
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| 5 |
1.583 |
1.543 |
1.563 |
1.417 |
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| 2.5 |
0.951 |
1.005 |
0.978 |
0.832 |
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| 1.25 |
0.602 |
0.637 |
0.620 |
0.474 |
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| 0.625 |
0.481 |
0.466 |
0.474 |
0.328 |
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| 0.312 |
0.291 |
0.304 |
0.298 |
0.152 |
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| 0.156 |
0.212 |
0.217 |
0.215 |
0.069 |
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| 0 |
0.148 |
0.144 |
0.146 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
7-14 working days |
