Human tartrate-resistant acid phosphatase 5b,TRACP-5b ELISA Kit
Product Details
| Target Name |
acid phosphatase 5, tartrate resistant |
| Alternative Names |
phosphatase ELISA Kit; Acid phosphatase 5 tartrate resistant ELISA Kit; Acid phosphatase 5; tartrate resistant ELISA Kit; ACP5 ELISA Kit; EC 3.1.3.2 ELISA Kit; MGC117378 ELISA Kit; PPA5_HUMAN ELISA Kit; serum band 5 tartrate-resistant acid phosphatase ELISA Kit; SPENCDI ELISA Kit; T5ap ELISA Kit; Tartrate resistant acid ATPase ELISA Kit; Tartrate resistant acid phosphatase type 5 ELISA Kit; Tartrate resistant acid phosphatase type 5 precursor ELISA Kit; Tartrate-resistant acid ATPase ELISA Kit; Tartrate-resistant acid phosphatase type 5 ELISA Kit; TR AP ELISA Kit; TR-AP ELISA Kit; TRACP 5 ELISA Kit; TRAP ELISA Kit; TrATPase ELISA Kit; Type 5 acid phosphatase ELISA Kit |
| Abbreviation |
ACP5 |
| Species |
Homo sapiens (Human) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.312 mIU/mL-20 mIU/mL |
| Sensitivity |
0.078 mIU/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human TRACP-5b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
83-92 |
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| 1:2 |
Average % |
99 |
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| Range % |
94-102 |
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| 1:4 |
Average % |
105 |
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| Range % |
100-108 |
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| 1:8 |
Average % |
95 |
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| Range % |
90-98 |
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| Recovery |
| The recovery of human TRACP-5b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
92-97 |
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| EDTA plasma (n=4) |
96 |
92-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| mIU/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.484 |
2.456 |
2.470 |
2.357 |
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| 10 |
1.918 |
1.876 |
1.897 |
1.784 |
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| 5 |
1.262 |
1.251 |
1.257 |
1.144 |
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| 2.5 |
0.666 |
0.715 |
0.691 |
0.578 |
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| 1.25 |
0.424 |
0.454 |
0.439 |
0.326 |
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| 0.625 |
0.312 |
0.329 |
0.321 |
0.208 |
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| 0.312 |
0.199 |
0.208 |
0.204 |
0.091 |
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| 0 |
0.111 |
0.115 |
0.113 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
